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Proteasome 20S α6 subunit monoclonal antibody (MCP20)

BML-PW8100-0025 25 µl 156.00 USD
BML-PW8100-0100 100 µl 431.00 USD
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Product Details

Alternative Name:PROS30, Proteasome subunit α type-1, Macropain subunit C2, Proteasome ν chain
Immunogen:Human placenta derived proteasomes.
UniProt ID:P25786
Gene/Protein Identifier:PSMA1 (gene name)
Source:Purified from ascites.
Species reactivity:Human
Specificity:Recognizes the α6 subunit of proteasome 20S.
Crossreactivity:Does not cross-react with rat or mouse protein.
Applications:IHC, IP, WB
Application Notes:Immunoprecipitation: This antibody has been immobilised on CNBr-activated Sepharose CL-4B and used to precipitate proteasomes from HeLa cells. Proteasomal subunits were subsequently separated by 2D PAGE.
Western Blot: Single dimension SDS-PAGE of a human placental proteasome preparation or HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of approximately 33kDa.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS containing 10mM sodium azide.
Use/Stability:Aliquot undiluted antibody into smaller volumes (not less than 10µL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-mouse IgG linker antibody is to be used).
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Technical Info/Product Notes:Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.
Regulatory Status:RUO - Research Use Only

Product Literature References

Functional and quantitative evaluation of the 20S proteasome in serum and intracellular in145 moroccan patients with hematologic malignancies: H. Filali, et al.; Clin. Proteomics 19, 41 (2022), Abstract;
Proteasome activation enhances stemness and lifespan of human mesenchymal stem cells: M. Kapetanou, et al.; Free Radic. Biol. Med. 103, 226 (2017), Abstract;
The proteasome maturation protein POMP increases proteasome assembly and activity in psoriatic lesional skin: B.A. Zieba, et al.; J. Dermatol. Sci. 88, 10 (2017), Abstract;
Circulating 20S Proteasome Is Independently Associated with Abdominal Muscle Mass in Hemodialysis Patients: H. Fukasawa, et al.; PLoS One 10, e0121352 (2015), Application(s): Western Blot, Abstract; Full Text
Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE): D.S. Pitcher, et al.; Biochim. Biophys. Acta 1844, 2222 (2014), Abstract;
TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions: Z. Lukic, et al.; Retrovirology 8, 93 (2011), Abstract; Full Text

General Literature References

Mobilization of MHC class I molecules from late endosomes to the cell surface following activation of CD34-derived human Langerhans cells: P.A. MacAry, et al.; PNAS 98, 3982 (2001), Abstract;
Bax degradation by the ubiquitin/proteasome-dependent pathway: involvement in tumor survival and progression: B. Li & Q.P. Dou; PNAS 97, 3850 (2000), Abstract;
Degradation of transcription factor RFX5 during the inhibition of both constitutive and interferon gamma-inducible major histocompatibility complex class I expression in chlamydia-infected cells: G. Zhong, et al.; J. Exp. Med. 191, 1525 (2000), Abstract;
Human proteasomes analysed with monoclonal antibodies: K.B. Hendil, et al.; Biochem. J. 305 ( Pt 1), 245 (1995), Abstract;

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