Product Details
| Alternative Name: | PROS30, Proteasome subunit α type-1, Macropain subunit C2, Proteasome ν chain |
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| Clone: | MCP20 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Human placenta derived proteasomes. |
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| UniProt ID: | P25786 |
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| Gene/Protein Identifier: | PSMA1 (gene name) |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human
Rabbit
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| Specificity: | Recognizes the α6 subunit of proteasome 20S. |
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| Crossreactivity: | Does not cross-react with rat or mouse protein. |
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| Applications: | IHC, IP, WB
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| Application Notes: | Immunoprecipitation: This antibody has been immobilised on CNBr-activated Sepharose CL-4B and used to precipitate proteasomes from HeLa cells. Proteasomal subunits were subsequently separated by 2D PAGE.
Western Blot: Single dimension SDS-PAGE of a human placental proteasome preparation or HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of approximately 33kDa. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 10mM sodium azide. |
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| Use/Stability: | Aliquot undiluted antibody into smaller volumes (not less than 10µL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-mouse IgG linker antibody is to be used). |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Proteasome activation enhances stemness and lifespan of human mesenchymal stem cells: M. Kapetanou, et al.; Free Radic. Biol. Med.
103, 226 (2017),
Abstract;
The proteasome maturation protein POMP increases proteasome assembly and activity in psoriatic lesional skin: B.A. Zieba, et al.; J. Dermatol. Sci.
88, 10 (2017),
Abstract;
Circulating 20S Proteasome Is Independently Associated with Abdominal Muscle Mass in Hemodialysis Patients: H. Fukasawa, et al.; PLoS One
10, e0121352 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE): D.S. Pitcher, et al.; Biochim. Biophys. Acta
1844, 2222 (2014),
Abstract;
TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions: Z. Lukic, et al.; Retrovirology
8, 93 (2011),
Abstract;
Full Text
General Literature References
Mobilization of MHC class I molecules from late endosomes to the cell surface following activation of CD34-derived human Langerhans cells: P.A. MacAry, et al.; PNAS
98, 3982 (2001),
Abstract;
Bax degradation by the ubiquitin/proteasome-dependent pathway: involvement in tumor survival and progression: B. Li & Q.P. Dou; PNAS
97, 3850 (2000),
Abstract;
Degradation of transcription factor RFX5 during the inhibition of both constitutive and interferon gamma-inducible major histocompatibility complex class I expression in chlamydia-infected cells: G. Zhong, et al.; J. Exp. Med.
191, 1525 (2000),
Abstract;
Human proteasomes analysed with monoclonal antibodies: K.B. Hendil, et al.; Biochem. J.
305 ( Pt 1), 245 (1995),
Abstract;
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