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Nbr1 (human) polyclonal antibody (fluorescein labeled)

BML-PW1130-0025 25 µl 159.00 USD
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For selective autophagy and protein aggregation studies using fluorescence microscopy.

Product Specification

Immunogen:Synthetic peptide corresponding to aa 38-51 of human Nbr1.
UniProt ID:Q14596
Source:Purified from rabbit serum.
Species reactivity:Human
Recommended Dilutions/Conditions:Immunocytochemistry (1:20)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Partially purified by salt precipitation.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C. Reagent is light sensitive; store in the dark. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended.
Handling:Protect from light. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:Autophagy is a highly conserved bulk protein degradation pathway responsible for the turnover of long-lived proteins, disposal of damaged organelles, and clearance of aggregate-prone proteins. Autophagy is involved in various physiological or pathological processes, such as development, host defense response, cancer and neuronal degenerative diseases. It involves concerted action of more than 20 specific autophagy (ATG) proteins that mediate the formation of a double-membrane vesicle, the autophagosome, which engulfs the substrate and delivers it to the lysosome for degradation.

Nbr1 (neighbor of BRCA1 gene 1) has been identified, together with p62, as a selective autophagy receptor, capable of simultaneously binding both ubiquitin and autophagy-specific LC3/GABARAP proteins. Nbr1 is recruited to ubiquitin-positive protein aggregates and degraded by autophagy through its interaction with the LC3/GABARAP component of the autophagosomal membrane.

Such receptor proteins provide a molecular link between ubiquitinylation and autophagy, facilitating the selective targeting of various types of cargo, ranging from protein aggregates to membrane-bound organelles and microbes for autophagic degradation.
Technical Info/Product Notes:Nbr1 polyclonal antibody was conjugated to fluorescein by a proprietary method. The fluorescein-labelled Nbr1 polyclonal antibody (Prod. No. BML-PW1130) facilitates ready detection of Nbr1 in autophagy inhibited cells by fluorescence microscopy.
Regulatory Status:RUO - Research Use Only
Nbr1 (human) polyclonal antibody (fluorescein labeled) Immunofluorescence
Detection of Nbr1 in HeLa cells by fluorescence microscopy using fluorescein labeled Nbr1 pAb (BML-PW1130), at 1:20 dilution. HeLa cells were pre-treated with autophagy inhibitor bafilomycin (BML-CM110) before analysis.
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Nbr1 (human) polyclonal antibody (fluorescein labeled) Immunofluorescence

General Literature References

A role for NBR1 in autophagosomal degradation of ubiquitinated substrates: V. Kirkin, et al.; Mol. Cell. 33, 505 (2009), Abstract;
A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol.Cell. 34, 259 (2009), Abstract;
NBR1 and p62 as cargo receptors for selective autophagy of ubiquitinated targets: T. Lamark, et al.; Cell Cycle 8, 1986 (2009), Abstract;
Autophagy fights disease through cellular self-digestion: M. Mizushima, et al.; Nature 451, 1069 (2008), Abstract;
Autophagosome formation: core machinery and adaptations: Z. Xie, et al.; Nat. Cell. Biol. 9, 1102 (2007), Abstract;
Autophagy in the eukaryotic cell: F. Reggiori, et al.; Eukaryot. Cell. 1, 11 (2002), Abstract;

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