| BML-PW1020-0025 | 25 µl | 143.00 USD |  |
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| BML-PW1020-0100 | 100 µl | 453.00 USD | |
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| Do you need bulk/larger quantities? | |||||
| Alternative Name: | Proteasome subunit β type-11 |
| Clone: | b8-17 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Recombinant human 20S proteasome β5t subunit. |
| UniProt ID: | A5LHX3 |
| Gene/Protein Identifier: | PSMB11 (gene name) |
| Source: | Purified from hybridoma tissue culture supernatant. |
| Species reactivity: | Human, Mouse |
| Specificity: | Recognizes the β5t subunit of the 20S proteasome. |
| Applications: | WB |
| Recommended Dilutions/Conditions: | Western Blot (1:500-1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Purity Detail: | Ion exchange purified. |
| Formulation: | Liquid. In PBS containing 0.01% sodium azide. |
| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| Shipping: | Shipped on Blue Ice |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Scientific Background: | When cells are stimulated with pro-inflammatory cytokines, most of their constitutively expressed proteasomes are replaced with immunoproteasomes, which increase the production of peptides for presentation on MHC class I molecules. In addition, cortical thymic epithelial cells selectively express a type of proteasome known as the thymoproteasome, in which the β5i immunoproteasome subunit has been replaced by the thymus specific proteasome subunit β5t. The thymoproteasome is required for the positive selection of thymocytes and shaping the T cell receptor repertoire of cytotoxic T lymphocytes. In addition mice deficient in β5t display defective development of CD8(+) T cells in the thymus suggesting a key role for β5t and the thymoproteasome in generating MHC class I-restricted CD8(+) T cells. |
| Technical Info/Product Notes: | The hybridoma secreting the antibody clone b8-17 was generated by fusion of splenocytes from Balb/cmice which had received repeated immunisation with recombinant human β5t subunit protein (accession number: A5LHX3). Immunoglobulin was purified from tissue culture supernatant by ion exchange chromatography. |
| Regulatory Status: | RUO - Research Use Only |
