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Proteasome 20S β5t subunit (human) monoclonal antibody (b8-17)

BML-PW1020-0025 25 µl 147.00 USD
BML-PW1020-0100 100 µl 466.00 USD
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Product Details

Alternative Name:Proteasome subunit β type-11
Immunogen:Recombinant human 20S proteasome β5t subunit.
UniProt ID:A5LHX3
Gene/Protein Identifier:PSMB11 (gene name)
Source:Purified from hybridoma tissue culture supernatant.
Species reactivity:Human, Mouse
Specificity:Recognizes the β5t subunit of the 20S proteasome.
Recommended Dilutions/Conditions:Western Blot (1:500-1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Ion exchange purified.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:When cells are stimulated with pro-inflammatory cytokines, most of their constitutively expressed proteasomes are replaced with immunoproteasomes, which increase the production of peptides for presentation on MHC class I molecules. In addition, cortical thymic epithelial cells selectively express a type of proteasome known as the thymoproteasome, in which the β5i immunoproteasome subunit has been replaced by the thymus specific proteasome subunit β5t. The thymoproteasome is required for the positive selection of thymocytes and shaping the T cell receptor repertoire of cytotoxic T lymphocytes. In addition mice deficient in β5t display defective development of CD8(+) T cells in the thymus suggesting a key role for β5t and the thymoproteasome in generating MHC class I-restricted CD8(+) T cells.
Technical Info/Product Notes:The hybridoma secreting the antibody clone b8-17 was generated by fusion of splenocytes from Balb/cmice which had received repeated immunisation with recombinant human β5t subunit protein (accession number: A5LHX3). Immunoglobulin was purified from tissue culture supernatant by ion exchange chromatography.
Regulatory Status:RUO - Research Use Only
Proteasome 20S β5t subunit (human) monoclonal antibody (b8-17) Western blot
Western blot showing detection of 20S proteasome subunit β5t in C57/BL6 mouse thymus lysate by BML-PW1020 at 1:1000 dilution.
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Proteasome 20S β5t subunit (human) monoclonal antibody (b8-17) Western blot

General Literature References

Proteasomes in immune cells: more than peptide producers?: M. Groettrup, et al.; Nat. Rev. Immunol. 10, 73 (2010), Abstract;
Thymoproteasome shapes immunocompetent repertoire of CD8+ T cells: T. Nitta, et al.; Immunity 32, 29 (2010), Abstract;
Exclusive expression of proteasome subunit {beta}5t in the human thymic cortex : U. Tomaru, et al.; Blood 113, 5186 (2009), Abstract;
Regulation of CD8+ T cell development by thymus-specific proteasomes: S. Murata, et al.; Science 316, 1349 (2007), Abstract;

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