Product Specification
| Alternative Name: | Ubiquitin thioesterase 30, Ubiquitin-specific-processing protease 30, Deubiquitinating enzyme 30, Ub-specific protease 30 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 170-185 of human USP30. |
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| UniProt ID: | Q70CQ3 |
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| Source: | Whole rabbit serum. |
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| Species reactivity: | Human
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. Crude serum containing 0.1% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | USP30 (ubiquitin specific protease 30) is a 517 amino acid deubiquitinylating enzyme that localizes to the mitochondrion (embedded in the outer mitochondrial membrane). Depletion of USP30 expression by RNA interference induces elongated and interconnected mitochondria, without changing the expression levels of the key regulators for mitochondrial dynamics, suggesting a key role for USP30 in regulating mitochondrial morphology.
USP30 has also been shown to be necessary for certain aspects of the hepatocyte growth factor (HGF)-dependent scattering response of epithelial cells, indicating that USP30 could be a potential target for therapeutic intervention to prevent metastasis. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Detection of USP30 in human liver cell mitochondrial preparation (2.5µg) by Western blot using USP30 (human), pAb (Prod. No. BML-PW0975) (1:1’000).
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General Literature References
Deubiquitinase activities required for hepatocyte growth factor-induced scattering of epithelial cells: R. Buus et al.; Curr. Biol.
19, 1463 (2009),
Abstract;
Regulation of mitochondrial morphology by USP30, a deubiquitinating enzyme present in the mitochondrial outer membrane: N. Nakamura & S. Hirose; Mol. Biol. Cell.
19, 1903 (2008),
Abstract;
