Product Specification
| Alternative Name: | HECT domain and RCC1-like domain-containing protein 5, Cyclin-E-binding protein 1, CEBP1 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to amino acid residues 402-414 of human Herc5. |
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| UniProt ID: | Q9UII4 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human
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| Crossreactivity: | Does not cross-react with Herc6. |
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Partially purified by salt precipitation. |
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| Formulation: | Liquid. In PBS containing 0.01% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | ISG15 is an interferon-induced and antiviral ubiquitin-like protein (Ubl). The ability of ISG15 to be conjugated to other proteins has been shown in some cases to be essential for its antiviral activity. Herc5 (HECT domain and RCC1-like domain-containing protein 5), the major E3 enzyme for ISG15, mediates the ISGylation of more than 300 proteins in interferon-stimulated cells. Herc5 is thought to broadly modify newly synthesized proteins in a co-translational manner, suggesting that, in the context of an interferon-stimulated cell, newly translated viral proteins may be primary targets of ISG15. The biochemical function of ISG15 conjugation and the basis of the antiviral activities of ISG15 conjugation have yet to be fully elucidated. |
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| Technical Info/Product Notes: | BML-PW0920 has been characterized extensively by Western blotting of Herc5 transfected 293T cell lysate and IFN-stimulated 293T, HeLa and A549 cell lysates. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot detection of Herc5 in Herc5 transfected 293T cell lysate (1) and IFN stimulated 293T cell lysate (2: -IFN; 3: +IFN) using BML-PW0920 at 1:1000 dilution.
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Product Literature References
The ISG15 conjugation system broadly targets newly synthesized proteins: implications for the antiviral function of ISG15: L.A. Durfee, et al.; Mol. Cell
38, 722 (2010),
Abstract;
General Literature References
The ISG15 conjugation system broadly targets newly synthesized proteins: implications for the antiviral function of ISG15: L.A. Durfee, et al.; Mol. Cell
38, 722 (2010),
Abstract;
Interferon-induced ISG15 conjugation inhibits influenza A virus gene expression and replication in human cells: T.Y. Hsiang, et. al.; J. Virol.
83, 5971 (2009),
Abstract;
ISG15 Arg151 and the ISG15-conjugating enzyme UbE1L are important for innate immune control of Sindbis virus: N.V. Giannakopoulos, et al.; J. Virol.
83, 1602 (2009),
Abstract;
Identification and Herc5-mediated ISGylation of novel target proteins: T. Takeuchi, et al.; Biochem. Biophys. Res. Commun.
348, 473 (2006),
Abstract;
HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets: J.J. Wong, et al.; PNAS USA
103, 10735 (2006),
Abstract;
Herc5, an interferon-induced HECT E3 enzyme, is required for conjugation of ISG15 in human cells: A. Dastur, et al.; J. Biol .Chem.
281, 4334 (2006),
Abstract;
Innate antiviral response targets HIV-1 release by the induction of ubiquitin-like protein ISG15: A. Okumura, et. al.; PNAS USA
103, 1440 (2006),
Abstract;
Accumulation of an mRNA and protein in interferon-treated Ehrlich ascites tumour cells: P.J. Farrell, et al.; Nature
279, 523 (1979),
Abstract;
