Product Specification
| Alternative Name: | Myb-like, SWIRM and MPN domain-containing protein 1 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 665-679 of human MYSM1. |
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| UniProt ID: | Q5VVJ2 |
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| Species reactivity: | Human
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Purified by salt precipitation. |
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| Formulation: | Liquid. In PBS containing 0.01% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | MYSM1 (Myb-like, SWIRM and MPN domain-containing protein 1) is a JAMM domain containing zinc metalloprotease that acts a deubiquitinylating enzyme specific for mono-ubiquitinylated histone H2A. MYSM1 acts by coordinating histone acetylation and deubiquitination, and destabilizing the association of linker histone H1 with nucleosomes. In addition, MYSM1 participates in transcriptional regulation events in androgen receptor-dependent gene activation. Levels of monoubiquitinylated H2A are dramatically decreased in prostate tumors, suggesting MYSM1 expression may serve as a cancer marker. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Western blot detection of GFP-MYSM1 transfected cell lysate (20µg) at ~122kDa by PAb to MYSM1 (Prod. No.
BML-PW0660) at 1:1'000 dilution. Other bands seen are degradation products of the full-length protein and are abolished upon peptide blockade."
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General Literature References
A histone H2A deubiquitinase complex coordinating histone acetylation and H1 dissociation in transcriptional regulation: P. Zhu, et al.; Mol. Cell
27, 609 (2007),
Abstract;
The role of chromatin during transcription: B. Li, et al.; Cell
128, 707 (2007),
Abstract;
Histone H2B ubiquitylation controls processive methylation but not monomethylation by Dot1 and Set1: M.D. Shahbazian, et al.; Mol. Cell
19, 271 (2005),
Abstract;
Polycomb group proteins Ring1A/B link ubiquitylation of histone H2A to heritable gene silencing and X inactivation: M. de Napoles, et al.; Dev. Cell
7, 663 (2004),
Abstract;
Rad6 plays a role in transcriptional activation through ubiquitylation of histone H2B: C.F. Kao, et al.; Genes Dev.
18, 184 (2004),
Abstract;
Transcriptional regulation by histone ubiquitination and deubiquitination: Y. Zhang; Genes Dev.
17, 2733 (2003),
Abstract;
