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USP21 (human) polyclonal antibody

BML-PW0585-0025 25 µl 143.00 USD
BML-PW0585-0100 100 µl 471.00 USD
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Product Details

Alternative Name:Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, Deubiquitinating enzyme 21, NEDD8-specific protease
Immunogen:Synthetic peptide corresponding to aa 60-74 of human USP21.
UniProt ID:Q9UK80
Source:Purified from rabbit serum.
Species reactivity:Human
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Purified by salt precipitation.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:USP21 is a ubiquitin-specific protease that catalyzes the hydrolysis of ubH2A thereby acting to relieve ubH2A-specific repression. USP21 is also thought to be involved with hepatocyte regeneration. Furthermore, in addition to its deubiquitinylating activity, USP21 is capable of removing NEDD8 from NEDD8 conjugates but has no effect on SUMO conjugates.
Regulatory Status:RUO - Research Use Only
USP21 (human) polyclonal antibody Western blot

Figure: Detection of USP21 in HeLa nuclear fraction (10µg) by Western blotting using PAb to USP21 (human) (Prod. No. BML-PW0585) at 1:1'000 dilution. Note: The bands at ~40kDa are likely to be a truncated forms of the USP21 protein as both these and the full length USP21 band are removed by peptide blockade using the antigenic peptide.

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USP21 (human) polyclonal antibody Western blot

General Literature References

Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation: T. Nakagawa, et al.; Genes Dev. 22, 37 (2008), Abstract;
Chromatin modifications and their function: T. Kouzarides; Cell 128, 693 (2007), Abstract;
Identification of a novel isopeptidase with dual specificity for ubiquitin- and NEDD8-conjugated proteins: L. Gong, et al.; J. Biol. Chem. 275, 14212 (2000), Abstract;

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