Ubiquitin, mAb (EX-9) detects ubiquitin conjugates and free polyubiquitin chains.
Product Specification
Clone: | EX-9 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Enzymatically synthesised polyubiquitin chains. |
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UniProt ID: | P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52) |
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Source: | Purified from tissue culture supernatant. |
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Species reactivity: | Species independent
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Specificity: | Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and, to a lesser extent, free ubiquitin. |
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Applications: | WB
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Recommended Dilutions/Conditions: | Western Blot (a starting dilution of 1:500-1:1'000 is recommended)
Do not use milk as blocking solution. Use 1% BSA in an appropriate buffer instead.
In order to obtain optimal polyubiquitin lysine linkage independent reactivity immunoblotting membranes must be autoclaved prior to blocking/probing with Ubiquitin, mAb (EX-9). Omitting this step results in decreased reactivity with a slight preference for K63-linked polyubiquitin.
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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Purity Detail: | Ion exchange purified. |
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Formulation: | Liquid. In PBS containing 0.01% sodium azide. |
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Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | -20°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure 1: Ubiquitin detection using Ubiquitin, mAb (EX-9) (Prod. No. BML-PW0580). Method: Western blot of Poly-ubiquitin chains (Ub2-7) (K48-linked) (Prod. No. BML-UW8860) (500ng) and Poly-ubiquitin chains (Ub2-7) (K63-linked) (Prod. No. BML-UW9570) (500ng). The membrane was autoclaved and probed with Ubiquitin, mAb (EX-9) (Prod. No. BML-PW0580) at 1:1000 dilution.
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Product Literature References
Novel Cell- and Tissue-Based Assays for Detecting Misfolded and Aggregated Protein Accumulation Within Aggresomes and Inclusion Bodies: D. Shen, et al.; Cell Biochem. Biophys.
60, 173 (2011),
Abstract;
Full Text
General Literature References
A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol.Cell.
34, 259 (2009),
Abstract;
Proteasome-independent functions of ubiquitin in endocytosis and signaling: D. Mukhopadhyay & H. Riezman; Science
315, 201 (2007),
Abstract;
AK1 is a ubiquitin-dependent kinase of MKK and IKK.: C. Wang et al.; Nature
412, 346 (2001),
Abstract;
The ubiquitin-proteasome pathway: on protein death and cell life: A. Ciechanover; EMBO J.
17, 7151 (1998),
Abstract;
Full Text