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Ubiquitin monoclonal antibody (EX-9)

BML-PW0580-0025 25 µl 129.00 USD
BML-PW0580-0100 100 µl 423.00 USD
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Ubiquitin, mAb (EX-9) detects ubiquitin conjugates and free polyubiquitin chains.

Product Details

Immunogen:Enzymatically synthesised polyubiquitin chains.
UniProt ID:P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
Source:Purified from tissue culture supernatant.
Species reactivity:Species independent
Specificity:Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and, to a lesser extent, free ubiquitin.
Recommended Dilutions/Conditions:Western Blot (a starting dilution of 1:500-1:1'000 is recommended)
Do not use milk as blocking solution. Use 1% BSA in an appropriate buffer instead.
In order to obtain optimal polyubiquitin lysine linkage independent reactivity immunoblotting membranes must be autoclaved prior to blocking/probing with Ubiquitin, mAb (EX-9). Omitting this step results in decreased reactivity with a slight preference for K63-linked polyubiquitin.
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Ion exchange purified.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:-20°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
Ubiquitin monoclonal antibody (EX-9) Western blot
Figure 1: Ubiquitin detection using Ubiquitin, mAb (EX-9) (Prod. No. BML-PW0580). Method: Western blot of Poly-ubiquitin chains (Ub2-7) (K48-linked) (Prod. No. BML-UW8860) (500ng) and Poly-ubiquitin chains (Ub2-7) (K63-linked) (Prod. No. BML-UW9570) (500ng). The membrane was autoclaved and probed with Ubiquitin, mAb (EX-9) (Prod. No. BML-PW0580) at 1:1000 dilution.
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Ubiquitin monoclonal antibody (EX-9) Western blot

Product Literature References

Novel Cell- and Tissue-Based Assays for Detecting Misfolded and Aggregated Protein Accumulation Within Aggresomes and Inclusion Bodies: D. Shen, et al.; Cell Biochem. Biophys. 60, 173 (2011), Abstract; Full Text

General Literature References

A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol.Cell. 34, 259 (2009), Abstract;
Proteasome-independent functions of ubiquitin in endocytosis and signaling: D. Mukhopadhyay & H. Riezman; Science 315, 201 (2007), Abstract;
AK1 is a ubiquitin-dependent kinase of MKK and IKK.: C. Wang et al.; Nature 412, 346 (2001), Abstract;
The ubiquitin-proteasome pathway: on protein death and cell life: A. Ciechanover; EMBO J. 17, 7151 (1998), Abstract; Full Text

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