| BML-PW0580-0025 | 25 µl | 129.00 USD |  |
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| BML-PW0580-0100 | 100 µl | 423.00 USD | |
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| Clone: | EX-9 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Enzymatically synthesised polyubiquitin chains. |
| UniProt ID: | P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52) |
| Source: | Purified from tissue culture supernatant. |
| Species reactivity: | Species independent |
| Specificity: | Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and, to a lesser extent, free ubiquitin. |
| Applications: | WB |
| Recommended Dilutions/Conditions: | Western Blot (a starting dilution of 1:500-1:1'000 is recommended) Do not use milk as blocking solution. Use 1% BSA in an appropriate buffer instead. In order to obtain optimal polyubiquitin lysine linkage independent reactivity immunoblotting membranes must be autoclaved prior to blocking/probing with Ubiquitin, mAb (EX-9). Omitting this step results in decreased reactivity with a slight preference for K63-linked polyubiquitin. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Purity Detail: | Ion exchange purified. |
| Formulation: | Liquid. In PBS containing 0.01% sodium azide. |
| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| Shipping: | Shipped on Blue Ice |
| Short Term Storage: | -20°C |
| Long Term Storage: | -20°C |
| Regulatory Status: | RUO - Research Use Only |
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