Product Details
| Alternative Name: | Sentrin-specific protease 6, SUSP1, SUMO-1-specific protease 1 |
| |
| Host: | Sheep |
| |
| Immunogen: | Recombinant human SENP6 (aa 635-1112) corresponding to the catalytic domain. |
| |
| UniProt ID: | Q9GZR1 |
| |
| Species reactivity: | Human
|
| |
| Applications: | WB
|
| |
| Recommended Dilutions/Conditions: | Western Blot (1:10,000; ensure that blocking is performed with 5% dried Milk/PBS/0.05% Tween, not BSA/PBST)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
| |
| Purity Detail: | Purified by salt precipitation and immunodepleted for species that cross-react with other SENP family members. |
| |
| Formulation: | Liquid. In PBS containing 0.01% sodium azide. |
| |
| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. Store diluted antibody at +4°C, do not freeze and use within 1 month. |
| |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| |
| Shipping: | Blue Ice |
| |
| Short Term Storage: | +4°C |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | SENP6 exhibits a preference for SUMO-2 and SUMO-3 in deconjugation reactions and SUMO-2/SUMO-3 chains in particular, whereas it processes SUMO precursor proteins poorly. SENP6 depletion leads to SUMO-2 and SUMO-3 accumulation in PML nuclear bodies. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Detection of SENP6 in HeLa nuclear fraction by Western blotting using BML-PW0370 at 1:10000 dilution. Note: The band at ~55kDa is a non-specific band consistently observed in both SENP6 expressing and SENP6 knockdown cell lysates.
Please mouse over
General Literature References
A stress-dependent SUMO4 sumoylation of its substrate proteins: W. Wei, et al.; BBRC
375, 454 (2008),
Abstract;
Structure of the human SENP7 catalytic domain and poly-SUMO deconjugation activities for SENP6 and SENP7: C.D. Lima & D. Reverter; J. Biol. Chem.
283, 32045 (2008),
Abstract;
Concepts in sumoylation: a decade on: R. Geiss-Friedlander & F. Melchior; Nat. Rev. Mol. Cell Biol.
8, 947 (2007),
Abstract;
Modification in reverse: the SUMO proteases: D. Mukhopadhyay & M. Dasso; Trends Biochem. Sci.
32, 286 (2007),
Abstract;
SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia: J. Cheng, et al.; Cell
131, 584 (2007),
Abstract;
SUMO protease SENP1 induces isomerization of the scissile peptide bond: L. Shen, et al.; Nat. Struct. Mol. Biol.
13, 1069 (2006),
Abstract;
SUMO-specific proteases and the cell cycle. An essential role for SENP5 in cell proliferation: A. Di Bacco & G. Gill; Cell Cycle
5, 2310 (2006),
Abstract;
SUSP1 antagonizes formation of highly SUMO2/3-conjugated species: D. Mukhopadhyay, et al.; J. Cell Biol.
174, 939 (2006),
Abstract;
SUMO, ubiquitin’s mysterious cousin: S. Muller, et al.; Nat. Rev. Mol. Cell Biol.
2, 202 (2001),
Abstract;
