Replaces Prod. #: ALX-270-280
Highly specific, cell permeable, and irreversible 20S proteasome inhibitor. Inhibits ubiquitin proteasome pathway in cell culture (IC50=1µM). It has been suggested that the natural product lactacystin acts as a precursor for clasto-lactacystin β-lactone and that the latter is the sole species that interacts with the proteasome. Inhibits cathepsin A. Leads to a reduction of adipogenesis. Induces neurite growth and inhibits cell cycle progression.
Product Details
| Alternative Name: | Omuralide |
| |
| Formula: | C10H15NO4 |
| |
| MW: | 213.2 |
| |
| CAS: | 155975-72-7 |
| |
| Purity: | ≥95% (HPLC) |
| |
| Appearance: | White solid. |
| |
| Solubility: | Soluble in DMSO (25mg/ml) or acetonitrile. |
| |
| Shipping: | Ambient Temperature |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | Stable for at least 2 years after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 1 month at -80°C. |
| |
| Handling: | Protect from light. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Please mouse over
Product Literature References
Cytosolic PTEN-INDUCED PUTATIVE KINASE 1 is Stabilized By NF-κB Pathway and Promotes Non-Selective Mitophagy: G.G. Lim, et al.; J. Biol. Chem.
290, 16882 (2015),
Abstract;
Full Text
Protein carbonylation and aggregation precede neuronal apoptosis induced by partial glutathione depletion: A. Dasgupta, et al.; ASN Neuro
4, e00084 (2012),
Abstract;
Full Text
Effect of proteasome inhibitor clasto-lactacystin-beta-lactone on the proteome of the haloarchaeon Haloferax volcanii: P.A. Kirkland, et al.; Microbiology
153, 2271 (2007),
Abstract;
Inhibition of human preadipocyte proteasomal activity by HIV protease inhibitors or specific inhibitor lactacystin leads to a defect in adipogenesis, which involves matrix metalloproteinase-9: S. De Barros, et al.; J. Pharmacol. Exp. Ther.
320, 291 (2007),
Abstract;
Lactacystin inhibits cathepsin A activity in melanoma cell lines: L. Kozlowski, et al.; Tumour Biol.
22, 211 (2001),
Abstract;
Lactacystin, a proteasome inhibitor: discovery and its application in cell biology: H. Tomoda & S. Omura; Yakugaku Zasshi
120, 935 (2000), Review,
Abstract;
Separation of cathepsin A-like enzyme and the proteasome: evidence that lactacystin/beta-lactone is not a specific inhibitor of the proteasome: H. Ostrowska, et al.; Int. J. Biochem. Cell Biol.
32, 747 (2000),
Abstract;
Total synthesis and biological activity of lactacystin, omuralide and analogs: E.J. Corey & W.D. Li; Chem. Pharm. Bull.
47, 1 (1999),
Abstract;
Lactacystin, proteasome function, and cell fate: G. Fenteany & S.L. Schreiber; J. Biol. Chem.
273, 8545 (1998),
Abstract;
Full Text
Lactacystin and clasto-lactacystin beta-lactone modify multiple proteasome beta-subunits and inhibit intracellular protein degradation and major histocompatibility complex class I antigen presentation: A. Craiu, et al.; J. Biol. Chem.
272, 13437 (1997),
Abstract;
Full Text
Mechanistic studies on the inactivation of the proteasome by lactacystin in cultured cells: L.R. Dick, et al.; J. Biol. Chem.
272, 182 (1997),
Abstract;
Full Text
Mechanistic studies on the inactivation of the proteasome by lactacystin: a central role for clasto-lactacystin beta-lactone: L.R. Dick, et al.; J. Biol. Chem.
271, 7273 (1996),
Abstract;
Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin: G. Fenteany, et al.; Science
268, 726 (1995),
Abstract;
A beta-lactone related to lactacystin induces neurite outgrowth in a neuroblastoma cell line and inhibits cell cycle progression in an osteosarcoma cell line: G. Fenteany, et al.; Proc. Natl. Acad. Sci. USA
91, 3358 (1994),
Abstract;
