Cell-permeable, ethyl ester of E-64-c. The ester is hydrolyzed by intracellular esterases to produce E-64-c. Inhibits calpain and cathepsins B, H, and L. Inhibits degradation of autophagic cargo inside autophagolysosomes.
Product Specification
Alternative Name: | 2S,3S-trans-(Ethoxycarbonyloxirane-2-carbonyl)-L-leucine-(3-methylbutyl) amide, Aloxistatin |
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Formula: | C17H30N2O5 |
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MW: | 342.4 |
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CAS: | 88321-09-9 |
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Purity: | ≥98% (HPLC) |
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Appearance: | White solid. |
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Solubility: | Soluble in 100% ethanol (>10mg/ml) or DMSO (20mg/ml). |
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Shipping: | Ambient |
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Long Term Storage: | -20°C |
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Use/Stability: | Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 2 months. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Targeting the lysosome by an aminomethylated Riccardin D triggers DNA damage through cathepsin B‐mediated degradation of BRCA1: Y. Wang, et al.; J. Cell. Mol. Med.
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Full Text
Mutants of collagen-specific molecular chaperone Hsp47 causing osteogenesis imperfecta are structurally unstable with weak binding affinity to collagen: S. Ito, et al; Biochem. Biophys. Res. Commun.
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Application(s): Cell culture,
Abstract;
Proteomic analysis of silenced cathepsin B expression suggests non-proteolytic cathepsin B functionality: F.C. Sigloch, et al.; Biochem. Biophys. Acta
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Application(s): Cell culture; inhibition experiments on U2OS cell line (ATCC),
Abstract;
Fatty acids increase adiponectin secretion through both classical and exosome pathways: V. DeClercq, et al.; Biochim. Biophys. Acta
1851, 1123 (2015),
Application(s): Cell Culture,
Abstract;
Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease: J. Peters, et al.; Biochem. Biophys. Res. Commun.
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Abstract;
Full Text
Lipopolysaccaride induces autophagic signaling in macrophages via a TLR4, heme oxygenase-1 dependent pathway: P. Waitz, et al.; Autophagy
7, 315 (2011),
Application(s): Protease Inhibition for Western Blot samples,
Abstract;
Regulation of Cathepsin G Reduces the Activation of Proinsulin-Reactive T Cells from Type 1 Diabetes Patients: F. Zhou, et al.; PLoS One
6, e22815 (2011),
Application(s): Modulation of cultured T-Cells,
Abstract;
Full Text
Inhibition of calpain in intact platelets by the thiol protease inhibitor E-64d: E. B. McGowan et al.; Biochem. Biophys. Res. Commun.
158, 432 (1989),
Abstract;
Efficient synthetic method for ethyl (+)-(2S,3S)-3-[(S)-3-methyl- 1-(3-methylbutylcarbamoyl)butylcarbamoyl]-2-oxiranecarb oxylate (EST), a new inhibitor of cysteine proteinases: M. Tamai et al.; Chem. Pharm Bull. (Tokyo)
35, 1098 (1987),
Abstract;