E-64-D is a cell-permeable ethyl ester of E-64-C (Prod. No. BML-PI106). The ester is hydrolyzed by intracellular esterases to produce E-64-C that inhibits calpain and cathepsins B, F, H, K, and L. E-64-D inhibits degradation of autophagic cargo inside autophagolysosomes, especially when used in combination with pepstatin (Prod. No. ALX-260-085). E-64-D combined with camostat mesylate also inhibits entry of SARS-CoV-2 in cells in vitro.
Soluble in DMSO (20mg/ml) or 100% ethanol (>10mg/ml).
Shipping:
Ambient Temperature
Long Term Storage:
-20°C
Use/Stability:
Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 2 months.
Regulatory Status:
RUO - Research Use Only
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Product Literature References
LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization: T. Tanaka, et al.; Autophagy 18, 1629 (2022), Abstract; Full Text
Lysosomal ceramides regulate cathepsin B-mediated processing of saposin C and glucocerebrosidase activity: M.J. Kim, et al.; Hum. Mol. Genet. 31, 2424 (2022), Abstract;
Phagosomal signalling of the C-type lectin receptor Dectin-1 is terminated by intramembrane proteolysis: T. Mentrup, et al.; Nat. Commun. 13, 1880 (2022), Abstract;
SerpinB3 drives cancer stem cell survival in glioblastoma: A. Lauko, et al.; Cell Rep. 40, 111348 (2022), Abstract; Full Text
The spike-stabilizing D614G mutation interacts with S1/S2 cleavage site mutations to promote the infectious potential of SARS-CoV-2 variants: S. Gellenoncourt, et al.; J. Virol. 96, e0130122 (2022), Abstract; Full Text
Trehalose Reduces the Secreted Beta-Amyloid Levels in Primary Neurons Independently of Autophagy Induction: I. Benito-Cuesta, et al.; Metabolites 11, 421 (2021), Abstract;
1-Deoxysphingolipids cause autophagosome and lysosome accumulation and trigger NLRP3 inflammasome activation: M. Lauterbach, et al.; Autophagy 17, 1947 (2021), Abstract; Full Text
Inhibition of striatonigral autophagy as a link between cannabinoid intoxication and impairment of motor coordination: C. Blázquez, et al.; Elife 9, 5681 (2021), Abstract; Full Text
Interleukin-1α is a central regulator of leukocyte-endothelial adhesion in myocardial infarction and in chronic kidney disease: S.J. Schunk, et al.; Circulation 144, 893 (2021), Abstract;
NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly: T. Niu, et al.; Nat. Commun. 12, 5862 (2021), Abstract;
Retrograde mitochondrial transport Is essential for organelle distribution and health in Zebrafish neurons: A. Mandal, et al.; J. Neurosci. 41, 1371 (2021), Abstract; Full Text
A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol: E. Fessler, et al.; Nature 579, 433 (2020), Abstract; Full Text
AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy: M. Dohmen, et al.; Nat. Commun. 11, 1032 (2020), Abstract; Full Text
BK Polyomavirus evades innate immune sensing by disrupting the mitochondrial network and promotes mitophagy: J. Manzetti, et al.; iScience 23, 101257 (2020), Abstract; Full Text
Diesel exhaust extract exposure induces neuronal toxicity by disrupting autophagy: L. Barnhill, et al.; Toxicol. Sci. 176, 193 (2020), Abstract; Full Text
Targeting the lysosome by an aminomethylated Riccardin D triggers DNA damage through cathepsin B‐mediated degradation of BRCA1: Y. Wang, et al.; J. Cell. Mol. Med. 23, 1798 (2019), Abstract; Full Text
Mutants of collagen-specific molecular chaperone Hsp47 causing osteogenesis imperfecta are structurally unstable with weak binding affinity to collagen: S. Ito, et al; Biochem. Biophys. Res. Commun. 469, 437 (2016), Application(s): Cell culture, Abstract;
Proteomic analysis of silenced cathepsin B expression suggests non-proteolytic cathepsin B functionality: F.C. Sigloch, et al.; Biochem. Biophys. Acta 1863, 2700 (2016), Application(s): Cell culture; inhibition experiments on U2OS cell line (ATCC), Abstract;
Fatty acids increase adiponectin secretion through both classical and exosome pathways: V. DeClercq, et al.; Biochim. Biophys. Acta 1851, 1123 (2015), Application(s): Cell Culture, Abstract;
Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease: J. Peters, et al.; Biochem. Biophys. Res. Commun. 457, 334 (2015), Application(s): Cell Culture, Abstract;
Mast Cell Subsets and Their Functional Modulation by the Acanthocheilonema viteae Product ES-62: D.H. Ball, et al.; J. Parasitol. Res. 2013, 961268 (2013), Abstract; Full Text
Lipopolysaccaride induces autophagic signaling in macrophages via a TLR4, heme oxygenase-1 dependent pathway: P. Waitz, et al.; Autophagy 7, 315 (2011), Application(s): Protease Inhibition for Western Blot samples, Abstract;
Regulation of Cathepsin G Reduces the Activation of Proinsulin-Reactive T Cells from Type 1 Diabetes Patients: F. Zhou, et al.; PLoS One 6, e22815 (2011), Application(s): Modulation of cultured T-Cells, Abstract; Full Text
Inhibition of calpain in intact platelets by the thiol protease inhibitor E-64d: E. B. McGowan et al.; Biochem. Biophys. Res. Commun. 158, 432 (1989), Abstract;
Efficient synthetic method for ethyl (+)-(2S,3S)-3-[(S)-3-methyl- 1-(3-methylbutylcarbamoyl)butylcarbamoyl]-2-oxiranecarb oxylate (EST), a new inhibitor of cysteine proteinases: M. Tamai et al.; Chem. Pharm Bull. (Tokyo) 35, 1098 (1987), Abstract;
