Replaces Prod. #: ALX-260-092
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High purity peptide
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Most economical MG-132 on the market
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Widely cited for more than two decades
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Well-characterized with a variety of applications
MG-132 is a potent, cell permeable and selective proteasome inhibitor (Ki = 4nM).1 It inhibits NF-κB activation by preventing IκB degradation (IC50 = 3μM). The peptide blocks degradation of short-lived proteins, which in turn induces HSP and ER chaperone expression, leading to thermotolerance (1μM MG-132, 2 h.). It also stimulates neurite outgrowth in PC12 cells (20nM optimal). The peptide has also been reported to increase the survival rate of mesenchymal stem cells following their transplantation. IC50’s for inhibition of Suc-LLVY-AMC and Z-LLL-AMC cleaving activities of proteasome were 0.85 and 0.1μM respectively. The ubiquitin-proteasome system (UPS) and autophagy serve as two complementary, reciprocally regulated protein degradation systems, thus blockade of UPS by MG-132 activates autophagy.
Product Details
| Alternative Name: | Z-LLL-CHO |
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| Sequence: | Z-Leu-Leu-Leu-CHO |
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| Formula: | C26H41N3O5 |
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| MW: | 475.6 |
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| CAS: | 133407-82-6 |
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| Purity: | ≥98% |
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| Appearance: | White solid. |
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| Solubility: | Soluble in DMSO (25mg/ml) or 100% ethanol (25mg/ml). |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Solutions are stable for up to one week if stored at -20°C. Solutions are stable for up to two months if stored at -80°C. |
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| Technical Info/Product Notes: | Replacement for ADI-HPK-116 |
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| Regulatory Status: | RUO - Research Use Only |
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A tumor suppressor Retinoblastoma1 is essential for embryonic development in the sea urchin: A. Fernandez-Nicolas, et al.; Dev. Dyn.
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Loss of periodontal ligament fibroblasts by RIPK3-MLKL-mediated necroptosis in the progress of chronic periodontitis: B.S. Han, et al.; Sci. Rep.
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Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases: E. Gatti; Methods Mol. Biol.
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The N-terminal sequence of murine PRMT5 variant 2 is required for Hsp70 interaction and CHIP ligase-mediated degradation: W. Lu, et al.; Biochem. Biophys. Res. Commun.
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Ubiquitin-proteasome system participates in the de-aggregation of spermadhesin and DQH protein during boar sperm capacitation: M. Zigo, et al.; Reproduction
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Curcumin suppresses oncogenicity of human colon cancer cells by covalently modifying the cysteine 67 residue of SIRT1: Y.H. Lee, et al.; Cancer Lett.
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Interactive effects of osmotic stress and burrowing activity on protein metabolism and muscle capacity in the soft shell clam Mya arenaria: F. Haider, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol.
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Modifications of the 26S proteasome during boar sperm capacitation: M. Zigo, et al.; Cell Tissue Res.
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PERK-mediated translational control is required for collagen secretion in chondrocytes: S. Hisanaga, et al.; Sci. Rep.
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Vps3 and Vps8 control integrin trafficking from early to recycling endosomes and regulate integrin-dependent functions: C.T.H. Jonker, et al.; Nat. Commun.
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A leptospiral AAA+ chaperone-Ntn peptidase complex, HslUV, contributes to the intracellular survival of Leptospira interrogans in hosts and the transmission of leptospirosis: S.L. Dong, et al.; Emerg. Microbes Infect.
6, e105 (2017),
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Cullin 3SPOP ubiquitin E3 ligase promotes the poly-ubiquitination and degradation of HDAC6: Y. Tan, et al.; Oncotarget
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Intrinsic ubiquitin E3 ligase activity of histone acetyltransferase Hbo1 for estrogen receptor α: M. Iizuka, et al.; Proc. Jpn. Acad. Ser. B Phys. Biol. Sci.
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The stability of Fbw7a in M-phase requires its phosphorylation by PKC: S. Zitouni, et al.; PLoS One
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WIP promotes in-vitro invasion ability, anchorage independent growth and EMT progression of A549 lung adenocarcinoma cells by regulating RhoA levels: A. Salvi, et al.; Biochem. Biophys. Res. Commun.
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Adjustments of Protein Metabolism in Fasting Arctic Charr, Salvelinus alpinus: A.A. Cassidy, et al.; PLoS One
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Agonist-Mediated Activation of STING Induces Apoptosis in Malignant B Cells: C.A. Tang, et al.; Cancer Res.
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Copper diethyldithiocarbamate as an activator of Nrf2 in cultured vascular endothelial cells: T. Fujie, et al.; J. Biol. Inorg. Chem.
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Cytotoxicity and Proteasome Inhibition by Alkaloid Extract from Murraya koenigii Leaves in Breast Cancer Cells-Molecular Docking Studies: A. Ismail, et al.; J. Med. Food.
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Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL: R. Yosef, et al.; Nat. Commun.
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Glycan-binding F-box protein from Arabidopsis thaliana protects plants from Pseudomonas syringae infection: K. Stefanowicz, et al.; BMC Plant Biol.
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Inhibition of β-catenin signaling by phenobarbital in hepatoma cells in vitro: N. Groll, et al.; Toxicology
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LRRK2 interferes with aggresome formation for autophagic clearance: Y. Bang, et al.; Moll. Cell Neurosci.
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Mifepristone increases mRNA translation rate, triggers the unfolded protein response, increases autophagic flux, and kills ovarian cancer cells in combination with proteasome or lysosome inhibitors: L. Zhang, et al.; Mol. Oncol.
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Phosphorylation of CHIP at Ser20 by Cdk5 promotes tAIF-mediated neuronal death: C. Kim, et al.; Cell Death Differ.
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PIAS3 enhances the transcriptional activity of HIF-1α by increasing its protein stability: K. Nakagawa, et al. ; Biochem. Biophys. Res. Commun.
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Posttranscriptional control of NLRP3 inflammasome activation in colonic macrophages: A.A. Filardy, et al.; Mucosal Immunol.
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Proteasomal degradation of sphingosine kinase 1 and inhibition of dihydroceramide desaturase by the sphingosine kinase inhibitors, SKi or ABC294640, induces growth arrest in androgen-independent LNCaP-AI prostate cancer cells: M. McNaughton, et al.; Oncotarget
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A point mutation in the ubiquitin ligase RNF170 that causes autosomal dominant sensory ataxia destabilizes the protein and impairs inositol 1,4,5-trisphosphate receptor-mediated Ca2+ signaling: F.A. Wright, et al.; J. Biol. Chem.
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ADAM12 and ADAM17 are essential molecules for hypoxia-induced impairment of neural vascular barrier function: D. Cui, et al.; Sci. Rep.
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Cinnamic aldehyde suppresses hypoxia-induced angiogenesis via inhibition of hypoxia-inducible factor-1α expression during tumor progression: W.Y. Bae, et al.; Biochem. Pharmacol.
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D1 dopamine receptor stimulation impairs striatal proteasome activity in Parkinsonism through 26S proteasome disassembly: P. Barroso-Chinea, et al.; Neurobiol. Dis.
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Fatty acids increase adiponectin secretion through both classical and exosome pathways: V. DeClercq, et al.; Biochim. Biophys. Acta
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RGS19 Converts Iron Deprivation Stress into a Growth-Inhibitory Signal: J. Hwang, et al.; Biochem. Biophys. Res. Commun.
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Signal transduction and downregulation of C-MET in HGF stimulated low and highly metastatic human osteosarcoma cells: K. Husmann, et al.; Biochem. Biophys. Res. Commun.
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UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP: Y. Teng, et al.; Biochem. Pharmacol.
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Variable Processing and Cross-presentation of HIV by Dendritic Cells and Macrophages Shapes CTL Immunodominance and Immune Escape: J. Dinter, et al.; PLoS Pathog.
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Identification and characterization of RING-finger ubiquitin ligase UBR7 in mammalian spermatozoa: S. Zimmerman, et al.; Cell Tissue Res.
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Application(s): IF, WB of boar, mouse and human sperm ,
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Kizuna is a novel mitotic substrate for CDC25B phosphatase: Y. Thomas, et al.; Cell Cycle
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Pivotal role for ROS activation of p38 MAPK in the control of differentiation and tumor-initiating capacity of glioma-initiating cells: A. Sato, et al.; Stem Cell Res.
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The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget
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A20 restricts wnt signaling in intestinal epithelial cells and suppresses colon carcinogenesis: L. Shao, et al.; PLoS One
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The ubiquitin proteasome system regulates the stability and activity of the glucose sensor glucokinase in pancreatic beta cells: A. Hofmeister-Brix, et al.; Biochem. J.
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Autophagy plays a protective role in endoplasmic reticulum stress-mediated pancreatic β cell death: A. Bartolome, et al.; Autophagy
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Full Text
Enhanced protein repair and recycling are not correlated with longevity in 15 vertebrate endotherm species: K.D. Salway, et al.; Age
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Novel Cell- and Tissue-Based Assays for Detecting Misfolded and Aggregated Protein Accumulation Within Aggresomes and Inclusion Bodies: D. Shen, et al.; Cell Biochem. Biophys.
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Full Text
SUMO E3 ligase activity of TRIM proteins: Y. Chu & X. Yang; Oncogene
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Application(s): Mammalian in vitro cells analyzed by WB,
Abstract;
Full Text
Induction of autophagy by proteasome inhibitor is associated with proliferative arrest in colon cancer cells: W.K. Wu, et al.; BBRC
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The human cytomegalovirus US11 gene product dislocates MHC class I heavy chains from the endoplasmic reticulum to the cytosol: E.J. Wiertz, et al.; Cell
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