Replaces Prod. #: ALX-167-025
Inactive control peptide for the cell-permeable NF-κB inhibitory peptide SN50. Contains the nuclear localization sequence (NLS residues 360-369) of the transcription factor NF-κB p50 linked to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF), except this control peptide has Lys363 to Asn and Arg364 to Gly. In mouse endothelial LE-II cells induced with LPS, SN50M peptide had no measureable effect on NF-κB translocation at 50 µg/ml. In human monocytic THP-1 cells induced by LPS or TNFα, there was slight inhibition of nuclear transport at 100 µg/ml. Sold under license from Vanderbilt University.
Product Details
| Sequence: | H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Gln-Arg-Asn-Gly-Gln-Lys-Leu-Met-Pro-OH |
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| Formula: | C123H215N33O30S |
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| MW: | 2668.0 |
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| Formulation: | Lyophilized. Counter Ion: Trifluoroacetate salt. |
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| Purity: | ≥97% (HPLC) |
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| Appearance: | White to off-white solid. |
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| Solubility: | Soluble in water. |
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| Reconstitution: | Reconstitute in sterile, distilled water. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Peptides in solution have limited stability. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
PML-Nuclear Bodies Regulate the Stability of the Fusion Protein Dendra2-Nrf2 in the Nucleus: A.F. Burroughs, et al.; Cell. Physiol. Biochem.
47, 800 (2019),
Abstract;
Full Text
The poly(ADP-ribose) polymerase inhibitor olaparib induces up-regulation of death receptors in primary acute myeloid leukemia blasts by NF-κB activation: I. Faraoni, et al.; Cancer Lett.
423, 127 (2018),
Abstract;
Transcriptional Regulation of the Sodium-activated Potassium Channel SLICK (KCNT2) promoter by nuclear factor-kappa B: D.L. Tomasello, et al.; J. Biol. Chem.
290, 18575 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
GDP-mannose-4,6-dehydratase (GMDS) deficiency renders colon cancer cells resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor- and CD95-mediated apoptosis by inhibiting complex II formation: K. Moriwaki, et al.; J. Biol. Chem.
286, 43123 (2011),
Abstract;
Full Text
Interruption of the NF-kappaB pathway by Bay 11-7082 promotes UCN-01-mediated mitochondrial dysfunction and apoptosis in human multiple myeloma cells.: Y. Dai et al.; Blood
103, 2761 (2004),
Abstract;
Full Text
Inhibition of nuclear translocation of transcription factor NF-kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence.: Y. Z. Lin et al.; J. Biol. Chem.
270, 14255 (1995),
Abstract;
