Replaces Prod. #: ALX-260-063
Colorimetric substrate for caspase-6 (Km=30µM; Kcat/Km=168’000 M-1sec-1) and related cysteine proteases. Sequence is based on the lamin A site of cleavage. Cleavage of the substrate by a caspase releases pNA, which can be detected by its absorbance at 405nm (ε=10,500 M-1cm-1).
Product Details
| Alternative Name: | Caspase-6 substrate (chromogenic) |
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| Sequence: | N-acetyl-Val-Glu-Ile-Asp-pNA (p-nitroanilide) |
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| MW: | 636.6 |
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| Source: | Synthetic. |
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| Formulation: | Lyophilized. |
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| Purity: | ≥95% (HPLC) |
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| Identity: | Determined by MS. |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble at 1 mg/ml in water with 5% ammonium hydroxide. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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General Literature References
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis.: N.A. Thornberry et al.; J. Biol. Chem.
272, 17907 (1997),
Abstract;
Substrate specificities of caspase family proteases.: R.V. Talanian et al.; J. Biol. Chem.
272, 9677 (1997),
Abstract;
The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32.: S.M. Srinivasula et al.; J. Biol. Chem.
271, 27099 (1996),
Abstract;
