This sequence, KKHTDDGYMPMSPGVA, is from insulin receptor substrate-1 (IRS-1) inclusive of Tyr608 (mouse) -Tyr612 (human). It contains the insulin receptor tyrosine kinase substrate motif YMXM (Tyr-Met-X-Met). This peptide has been used as a substrate for purified insulin receptor (Km=90 µM) and other tyrosine kinases in phosphocellulose binding assays. The tyrosine phosphorylated version of this peptide binds to phosphatidylinositol 3-kinase (PI 3-kinase) SH2 domain and activates the enzyme.
Product Details
| Alternative Name: | Insulin receptor substrate (Tyr608) peptide |
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| Sequence: | Lys-Lys-His-Thr-Asp-Asp-Gly-Tyr-Met-Pro-Met-Ser-Pro-Gly-Val-Ala |
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| MW: | 1732.8 |
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| Source: | Synthetic. |
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| Formulation: | Lyophilized. |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in water. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Regulation of phosphatidylinositol 3'-kinase by tyrosyl phosphoproteins. Full activation requires occupancy of both SH2 domains in the 85-kDa regulatory subunit: T. Rordorf-Nikolic, et al.; J. Biol. Chem.
270, 3662 (1995),
Abstract;
Phosphorylation of synthetic peptides containing Tyr-Met-X-Met motifs by nonreceptor tyrosine kinases in vitro: P. Garcia, et al.; J. Biol. Chem.
268, 25146 (1993),
Abstract;
YMXM motifs of IRS-1 define substrate specificity of the insulin receptor kinase: S.E. Shoelson, et al.; Proc. Natl. Acad. Sci. USA
89, 2027 (1992),
Abstract;
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