Highly sensitive substrate for human and mouse neutrophil elastase (leukocyte elastase) and neutrophil proteinase 3 (PR-3, myeloblastin), but not cathepsin G nor chymotrypsin. This substrate can be used for in vitro assays with purified enzyme, or with biological fluids or conditioned medium. Hydrolysis of the substrate can be detected at 405-410 nm.
Product Details
| Sequence: | N-Methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide |
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| MW: | 590.6 |
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| CAS: | 70967-90-7 |
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| Purity: | ≥98% |
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| Solubility: | Soluble in DMSO (>20 mM) or methanol (1 mg/mL). |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Deficiency of cathepsin C ameliorates severity of acute pancreatitis by reduction of neutrophil elastase activation and cleavage of E-cadherin: D.S. John, et al.; J. Biol. Chem.
294, 697 (2019),
Abstract;
Competitive neutrophil elastase inhibitory isoflavones from the roots of Flemingia philippinensis: J.Y. Kim, et al.; Bioorg. Chem.
78, 249 (2018),
Abstract;
The putative role of Rhipicephalus microplus salivary serpins in the tick-host relationship: L. Tirloni, et al.; Insect Biochem. Mol. Biol.
71, 12 (2016),
Application(s): Used for proteinase-3,
Abstract;
Engineering D-helix of antithrombin in alpha-1-proteinase inhibitor confers antiinflammatory properties on the chimeric serpin: L. Yang, et al.; Thromb. Haemost.
112, 164 (2014),
Abstract;
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