Replaces Prod. #: ALX-260-131
Fluorogenic substrate cleaved by plasma kallikrein and kallikrein 8, but not plasmin; by papain; and by most cathepsins (cathepsins B, C/DPP-I, F, K/O2, L, L2/V, O, S, and X/Z). Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144).
Product Details
| Alternative Name: | Z-FR-AMC, Z-Phe-Arg-AMC |
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| Sequence: | Z-FR-AMC, Z-Phe-Arg-AMC . HCl [Z=Cbz=Benzyloxycarbonyl; AMC=7-amino-4-methylcoumarin] |
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| Formula: | C33H36N6O6 . HCl |
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| MW: | 649.2 |
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| CAS: | 65147-22-0 |
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| Purity: | ≥99% |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cathepsin S Contributes to Lung Inflammation in Acute Respiratory Distress Syndrome: M.C. McKelvey, et al.; Am. J. Respir. Crit. Care Med.
1164, 1631OC (2022),
Abstract;
Modulation of Cystatin C in Human Macrophages Improves Anti-Mycobacterial Immune Responses to Mycobacterium tuberculosis Infection and Coinfection With HIV: D. Pires, et al.; Front. Immunol.
18, 742822 (2021),
Abstract;
Nrf2 activation induces mitophagy and reverses Parkin/Pink1 knock down-mediated neuronal and muscle degeneration phenotypes: S. Gumeni, et al.; Cell Death Dis.
12, 41419 (2021),
Abstract;
Biological Evaluation and In Silico Study of Benzoic Acid Derivatives from Bjerkandera adusta Targeting Proteostasis Network Modules: K. Georgousaki, et al.; Molecules
25, 666 (2020),
Application(s): Cathepsin B, L enzymatic activity in human foreskin fibroblasts (BJ) lysates.,
Abstract;
Full Text
Cell penetrable, clickable and tagless activity-based probe of human cathepsin L: D. Dana, et al.; Bioorg. Chem.
85, 505 (2019),
Application(s): Activity assay,
Abstract;
Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement: K. Gumpper, et al.; Methods Mol. Biol.
1854, 35 (2019),
Abstract;
Dynamic MTORC1-TFEB feedback signaling regulates hepatic autophagy, steatosis and liver injury in long-term nutrient oversupply: H. Zhang, et al.; Autophagy
14, 1779 (2018),
Abstract;
Indomethacin Disrupts Autophagic Flux by Inducing Lysosomal Dysfunction in Gastric Cancer Cells and Increases Their Sensitivity to Cytotoxic Drugs: J. Vallecillo-Hernandez, et al.; Sci. Rep.
8, 3593 (2018),
Abstract;
Full Text
Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients: C. Valdez, et al.; Hum. Mol. Genet.
26, 4861 (2017),
Abstract;
Full Text
Evaluation of trypanocidal activity of combinations of anti-sleeping sickness drugs with cysteine protease inhibitors: D. Steverding, et al.; Exp. Parasitol.
151, 28 (2015),
Application(s): Assay,
Abstract;
Development of a highly potent, selective, and cell-active Inhibitor of cysteine cathepsin L-A hybrid design approach: D. Dana, et al.; Chem. Commun. (Camb.)
50, 10875 (2014),
Abstract;
Cathepsin B is up-regulated and mediates extracellular matrix degradation in trabecular meshwork cells following phagocytic challenge: K. Porter, et al.; PLoS One
8, e68668 (2013),
Abstract;
Full Text
Suppression of lysosome function induces autophagy via a feedback down-regulation of MTOR complex 1 (MTORC1) activity: M. Li, et al.; J. Biol. Chem.
288, 35769 (2013),
Abstract;
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