Replaces Prod. #: ALX-260-151
Fluorogenic substrate for caspase-8 and granzyme B. Ex.: 360 nm, Em.: 450 nm. The Km of recombinant human granzyme B for Ac-IEPD-AMC is 160 µM, with a Vmax about eight-fold lower than that of Ac-IEPD-pNA (BML-P133).
Product Details
| Alternative Name: | Caspase-8 substrate (fluorogenic), Granzyme B substrate (fluorogenic) |
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| Sequence: | N-acetyl-Ile-Glu-Pro-Asp-AMC (7-amino-4-methylcoumarin) |
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| Formula: | C32H41N5O11 |
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| MW: | 671.8 |
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| Purity: | ≥95% |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stock solution in DMSO (e.g. 0.1M) is highly stable at -20°C or -70°C. |
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| Handling: | Keep cool and dry. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Definition and redesign of the extended substrate specificity of granzyme B: J.L. Harris, et al.; J. Biol. Chem.
273, 27364 (1998),
Abstract;
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem.
272, 17907 (1997),
Abstract;
