Sequence from site of phosphorylation by PKA on the ß-subunit of phosphorylase kinase. Highly specific and very potent substrate for cAMP-dependent protein kinase (PKA). PKI inhibited 90% substrate phosphorylation in various tissue extracts. Km=15 µM for PKA; 223 µM for PKG. Useful in measuring activity of this PKA via phosphorylation-induced decrease in fluorescence.
Product Specification
| Sequence: | H-Arg-Thr-Lys-Arg-Ser-Gly-Ser-Val-Tyr-Glu-Pro-Leu-Lys-Ile-OH |
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| MW: | 1633.8 |
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| CAS: | 86555-35-3 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in water. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Use of a synthetic dodecapeptide (malantide) to measure the cyclic AMP- dependent protein kinase activity ratio in a variety of tissues: K.J. Murray, et al.; Biochem. J.
267, 703 (1990),
Abstract;
Full Text
Characterization of a fluorescent substrate for the adenosine 3’,5’- cyclic monophosphate-dependent protein kinase: D.A. Malencik & S.R. Anderson; Anal. Biochem.
132, 34 (1983),
Abstract;
