This peptide was used to generate the guinea pig polyclonal antiserum to neurokinin-1 receptor (BML-NA4200). The peptide was synthesized using the Fmoc-polyamide mode of solid-phase synthesis. The peptide is suitable for use in negative control (antibody blocking) experiments in Western blotting and immunohistochemistry. The sequence exhibits a single amino acid substitution in the aligned sequence of mouse NK1R (M405I), two changes in the guinea pig NK1R sequence (M395V; A407S) and four changes in the human sequence (S399F; Y402S; M405V; A407S).
Product Details
| Sequence: | Lys-Thr-Met-Thr-Glu-Ser-Ser-Ser-Phe-Tyr-Ser-Asn-Met-Leu-Ala |
| |
| MW: | 1696.9 |
| |
| Purity: | ≥95% (HPLC) |
| |
| Appearance: | White powder. |
| |
| Solubility: | Soluble in water and aqueous buffers. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Differential activation of intracellular effector by two isoforms of human neurokinin-1 receptor: T.M. Fong, et al.; Mol. Pharmacol.
41, 24 (1992),
Abstract;
Human substance P receptor (NK-1): Organization of the gene, chromosome localization, and functional expression of cDNA
clones: N.P. Gerard, et al.; Biochemistry
30, 10640 (1991),
Abstract;
Molecular cloning, structural characterization and functional expression of the human substance P receptor: Y. Takeda, et al.; Biochem. Biophys. Res. Commun.
179, 1232 (1991),
Abstract;
Organization, structure, and expression of the gene encoding the rat substance P receptor: A.D. Hershey, et al.; J. Biol. Chem.
266, 4366 (1991),
Abstract;
Substance P (neurokinin-1) receptor mRNA is selectively expressed in cholinergic neurons in the striatum and basal forebrain: C.R. Gerfen, et al.; Brain Res.
556, 165 (1991),
Abstract;
Molecular characterization of a functional cDNA encoding the rat substance P receptor: A.D. Hershey & J.E. Krause; Science
247, 958 (1990),
Abstract;
Substance P receptors in the nervous system and possible receptor subtypes: L.L. Iversen, et al.; CIBA Found. Symp. 186-205 (1982),
Abstract;
