Replaces Prod. #: ALX-340-057
Primary metabolite of arachidonic acid via the 12-lipoxygenase pathway. Inhibits thromboxane synthase. Inhibits platelet aggregation via activation of guanylate cyclase (100% at 5µM). Stimulates leukotriene biosynthesis. Potent and selective inhibitor of Ca2+-calmodulin-dependent protein kinase II (IC50=0.7µM). Endogenous TRPV1 agonist (Ki=0.35µM).
Product Details
Alternative Name: | 12(S)-HPETE |
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Formula: | C20H32O4 |
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MW: | 336.5 |
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CAS: | 71774-10-2 |
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Concentration: | 50µg/ml |
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Formulation: | Solution in ethanol. |
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Purity: | ≥98% |
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Appearance: | Liquid. |
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Solubility: | Soluble in DMSO or dimethyl formamide; slightly soluble in PBS, pH 7.2 (0.8mg/ml) or 0.1M Na2CO3 (2mg/ml). |
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Shipping: | Shipped on Dry Ice |
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Long Term Storage: | -80°C |
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Use/Stability: | Stable for at least 6 months after receipt when stored at -80°C. Product is highly unstable in aqueous solution. We recommend using aqueous solutions within fifteen minutes.For experiments with cells or tissues, aliquots of ethanol solutions may be added |
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Handling: | After opening, prepare aliquots and store at -80°C. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Bradykinin-12-lipoxygenase-VR1 signaling pathway for inflammatory hyperalgesia: J. Shin, et al.; PNAS
99, 10150 (2002),
Abstract;
Direct activation of capsaicin receptors by products of lipoxygenases: endogenous capsaicin-like substances: S.W. Hwang, et al.; PNAS
97, 6155 (2000),
Abstract;
12-hydroperoxyeicosatetraenoic acid inhibits main platelet functions by activation of soluble guanylate cyclase: B. Brune & V. Ullrich; Mol. Pharmacol.
39, 671 (1991),
Abstract;
Inhibition of Ca2+/calmodulin-dependent protein kinase II by arachidonic acid and its metabolites: D. Piomelli, et al.; PNAS
86, 8550 (1989),
Abstract;
Stimulation of leukotriene biosynthesis in human blood leukocytes by platelet-derived 12-hydroperoxy-icosatetraenoic acid: J. Maclouf, et al.; PNAS
79, 6042 (1982),
Abstract;
Resolution of prostaglandin endoperoxide synthase and thromboxane synthase of human platelets: S. Hammarstrom & P. Falardeau; PNAS
74, 3691 (1977),
Abstract;