Replaces Prod. #: ALX-290-001
Members of the Ras superfamily of G proteins, γ-subunits of heterotrimeric G proteins and the α-subunit of cGMP phosphodiesterase from retinal rods are all carboxyl methylated at carboxy-terminal S-farnesylcysteine residues. AFC specifically inhibits the S-farnesylcysteine methyl transferase (Km=20 µM) in cell free extracts and in whole cells. It inhibits carboxyl methylation of p21ras platelet Rap15 and the γ-subunit of transducin. It blocks receptor-mediated signal transduction in platelets and neutrophils not by inhibiting the methyltransferase, but possibly by inhibiting capacitative Ca2+ entry.
Product Details
Alternative Name: | AFC |
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Formula: | C20H33NO3S |
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MW: | 367.5 |
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Source: | Synthetic. |
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CAS: | 135304-07-3 |
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Purity: | ≥98% (HPLC) |
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Appearance: | Pale yellow oil. |
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Solubility: | Soluble in DMSO (25mg/ml), methanol or 100% ethanol (25mg/ml). |
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Shipping: | Ambient |
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Long Term Storage: | -20°C |
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Handling: | Keep under inert gas. Protect from light. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Application(s): Cell culture,
Abstract;
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Abstract;
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Abstract;
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Abstract;
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Abstract;