Replaces Prod. #: ALX-290-001
Members of the Ras superfamily of G proteins, γ-subunits of heterotrimeric G proteins and the α-subunit of cGMP phosphodiesterase from retinal rods are all carboxyl methylated at carboxy-terminal S-farnesylcysteine residues. AFC specifically inhibits the S-farnesylcysteine methyl transferase (Km=20 µM) in cell free extracts and in whole cells. It inhibits carboxyl methylation of p21ras platelet Rap15 and the γ-subunit of transducin. It blocks receptor-mediated signal transduction in platelets and neutrophils not by inhibiting the methyltransferase, but possibly by inhibiting capacitative Ca2+ entry.
Product Details
| Alternative Name: | AFC |
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| Formula: | C20H33NO3S |
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| MW: | 367.5 |
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| Source: | Synthetic. |
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| CAS: | 135304-07-3 |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | Pale yellow oil. |
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| Solubility: | Soluble in DMSO (25mg/ml), methanol or 100% ethanol (25mg/ml). |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Handling: | Keep under inert gas. Protect from light. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Role of phosphatase activity of soluble epoxide hydrolase in regulating simvastatin-activated endothelial nitric oxide synthase: H. Hou, et al.; Sci. Rep.
5, 13524 (2015),
Application(s): Cell culture,
Abstract;
Inhibition of capacitative Ca2+ entry into cells by farnesylcysteine analogs: Y. Xu, et al.; Mol. Pharmacol.
50, 1495 (1996),
Abstract;
Farnesyl-L-cysteine analogs can inhibit or initiate superoxide release by human neutrophils: J. Ding, et al.; J. Biol. Chem.
269, 16837 (1994),
Abstract;
Carboxyl methylation of Ras-related proteins during signal transduction in neutrophils: M.R. Philips, et al.; Science
259, 977 (1993),
Abstract;
Protein prenylcysteine analog inhibits agonist-receptor-mediated signal transduction in human platelets: A. Huzoor, et al.; PNAS
90, 868 (1993),
Abstract;
Effects of farnesylcysteine analogs on protein carboxyl methylation and signal transduction: C. Volker, et al.; J. Biol. Chem.
266, 21515 (1991),
Abstract;
