Product Details
| Source: | From Vibrio cholerae. |
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| CAS: | 9012-63-9 |
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| UniProt ID: | P01555 (A subunit), P01556 (B subunit) |
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| Formulation: | Lyophilized from 0.05M Tris, pH 7.5, containing 200 mM NaCl, and 1 mM Na2 EDTA. |
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| Reconstitution: | Reconstitute with 0.5ml sterile water to a 2mg/mL solution. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | +4°C |
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| Handling: | Handle gently and do not vortex. Store at +4°C prior to and following reconstitution. Do not freeze. |
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| Scientific Background: | Cholera toxin consists of a single A subunit surrounded by five B subunits. The B subunits are responsible for the attachment of the native toxin to ganglioside GM1 on mammalian cell surfaces, and facilitate translocation of the A subunit. The A subunit catalyzes the ADP-ribosylation of an arginine residue on the a subunit of G proteins, reducing intrinsic GTPase activity and activating the a subunit. ADP-ribosylation of other proteins such as human red cell Ca-ATPase has also been reported. Cholera toxin must interact with ADP-ribosylation factor (ARF) for maximal activity. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A single N6-methyladenosine site regulates lncRNA HOTAIR function in breast cancer cells: A.M. Porman, et al.; PLoS Biol.
20, e3001885 (2022),
Abstract;
Caco-2/HT29-MTX co-cultured cells as a model for studying physiological properties and toxin-induced effects on intestinal cells: P. Hoffmann, et al.; PLoS One
16, e0257824 (2021),
Abstract;
Diallyl Sulfide Attenuation of Carcinogenesis in Mammary Epithelial Cells through the Inhibition of ROS Formation, and DNA Strand Breaks: S.F. Darling-Reed, et al.; Biomolecules
11, 1313 (2021),
Abstract;
