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ATP (Energy) Regeneration Solution

BML-EW9810-0100 100 µl 113.00 USD
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This solution contains MgCl2, ATP, and ATP regenerating enzymes to recycle ATP hydrolysis products, (AMP, ADP) to ATP. An ideal energy source for conjugation/degradation assays (ubiquitin, proteasome, SUMO, etc.) particularly those that are preformed with crude lysates/impure reagents that may contain ATP-depleting activities.

Product Details

Formulation:Liquid. In 50mM TRIS-Cl, pH 7.5.
Shipping:Dry Ice
Long Term Storage:-80°C
Use/Stability:It is a 10x solution.
Regulatory Status:RUO - Research Use Only

Product Literature References

A dimer-monomer switch controls CHIP-dependent substrate ubiquitylation and processing: V. Balaji, et al.; Mol. Cell 82, 3239 (2022), Abstract;
Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis: Y. Fan, et al.; Cell Death Differ. 29, 1673 (2022), Abstract;
Reduction in PA28αβ activation in HD mouse brain correlates to increased mHTT aggregation in cell models: K.W. Geijtenbeek, et al.; PLoS One 17, e0278130 (2022), Abstract;
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation: Y. Wu, et al.; Nat. Commun. 12, 2346 (2021), Abstract;
Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation: M. Ranes, et al.; Mol. Cell 81, 3246 (2021), Abstract;
The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover: R. Tawo, et al.; Cell 169, 470 (2017), Abstract; Full Text

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