This solution contains MgCl2, ATP, and ATP regenerating enzymes to recycle ATP hydrolysis products, (AMP, ADP) to ATP. An ideal energy source for conjugation/degradation assays (ubiquitin, proteasome, SUMO, etc.) particularly those that are preformed with crude lysates/impure reagents that may contain ATP-depleting activities.
Product Details
| Formulation: | Liquid. In 50mM TRIS-Cl, pH 7.5. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | It is a 10x solution. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis: Y. Fan, et al.; Cell Death Differ. (2022),
Abstract;
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation: Y. Wu, et al.; Nat. Commun.
12, 2346 (2021),
Abstract;
Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation: M. Ranes, et al.; Mol. Cell
81, 3246 (2021),
Abstract;
The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover: R. Tawo, et al.; Cell
169, 470 (2017),
Abstract;
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