Replaces Prod. #: ALX-270-339
Potent, cell permeable, selective, and reversible inhibitor of c-Jun N-terminal kinase (JNK) (IC50=40nM for JNK-1 and JNK-2 and 90nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38. Inhibits the phosphorylation of c-Jun and blocks the expression of IL-2, IFN-γ, TNF-α, and COX-2. Blocks IL-1-induced accumulation of phospho-Jun, induction of c-Jun transcription and anti CD3-induced apoptosis of CD4+ CD8+ thymocytes.
Product Details
| Alternative Name: | Anthra(1,9-cd)pyrazol-6(2H)-one, 1,9-Pyrazoloanthrone |
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| Formula: | C14H8N2O |
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| MW: | 220.2 |
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| CAS: | 129-56-6 |
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| RTECS: | CB4585000 |
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| Purity: | ≥98% (TLC) |
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| Appearance: | Yellow to brown solid. |
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| Solubility: | Soluble in DMSO or methanol. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | Ambient |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at +20°C. Stock solutions are stable for up to 3 months at -20°C. |
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| Handling: | Protect from light. Keep under inert gas. |
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| Technical Info/Product Notes: | Note: Product is not sterile.
Replacement for ADI-HPK-111. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Picolinafen exposure induces ROS accumulation and calcium depletion, leading to apoptosis in porcine embryonic trophectoderm and uterine luminal epithelial cells: W. Park, et al.; Theriogenology
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Aclonifen induces bovine mammary gland epithelial cell death by disrupting calcium homeostasis and inducing ROS production: J. Park, et al.; Pestic. Biochem. Physiol.
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Exosomal p38 Mitogen-activated Protein Kinase Promotes Tumour Repopulation in TP53-mutated Bile Duct Cancer Cells: M. Osawa, et al.; Anticancer Res.
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Hepatocyte Bcl-3 protects from death-receptor mediated apoptosis and subsequent acute liver failure: N. Gehrke, et al.; Cell Death Dis.
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Nobiletin Promotes Megakaryocytic Differentiation through the MAPK/ERK-Dependent EGR1 Expression and Exerts Anti-Leukemic Effects in Human Chronic Myeloid Leukemia (CML) K562 Cells: J.H. Yen, et al.; Cells
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The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties: K. Oniki, et al.; Sci. Rep.
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Anti-inflammatory effects of mesenchymal stem cell-derived exosomal microRNA-146a-5p and microRNA-548e-5p on human trophoblast cells: C. Yang, et al.; Mol. Hum. Reprod.
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Nemo-like kinase drives Foxp3 stability and is critical for maintenance of immune tolerance by regulatory T cells: V. Fleskens, et al.; Cell Rep.
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Quercetin augments apoptosis of canine osteosarcoma cells by disrupting mitochondria membrane potential and regulating PKB and MAPK signal transduction: S. Ryu, et al.; J. Cell. Biochem.
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Carbon monoxide releasing molecule-2 attenuates Pseudomonas aeruginosa-induced ROS-dependent ICAM-1 expression in human pulmonary alveolar epithelial cells: C.W. Lee, et al.; Redox Biol.
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Chrysin attenuates progression of ovarian cancer cells by regulating signaling cascades and mitochondrial dysfunction: W. Lim, et al.; J. Cell. Physiol.
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Fibroblast growth factor 2 induces proliferation and distribution of G2 /M phase of bovine endometrial cells involving activation of PI3K/AKT and MAPK cell signaling and prevention of effects of ER stress: W. Lim, et al.; J. Cell. Physiol.
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PRMT1 mediates RANKL-induced osteoclastogenesis and contributes to bone loss in ovariectomized mice: J.H. Choi, et al.; Exp. Mol. Med.
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Sideroxylin (Callistemon lanceolatus) suppressed cell proliferation and increased apoptosis in ovarian cancer cells accompanied by mitochondrialdysfunction, the generation of reactive oxygen species, and an increase of lipid peroxidation: S. Park, et al.; J. Cell. Physiol.
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Application(s): Treatment of ovarian cancer cells (ES2 and OV90),
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Differential expression and functional roles of chemokine (C-C motif) ligand 23 and its receptor chemokine (C-C motif) receptor type 1 in the uterine endometrium during early pregnancy in pigs: W. Jeong, et al.; Dev. Comp. Immunol.
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Endogenous AMPK acts as a detrimental factor in fulminant hepatitis via potentiating JNK-dependent hepatocyte apoptosis: K. Hu, et al.; Cell Death Dis.
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Gomisin N Decreases Inflammatory Cytokine Production in Human Periodontal Ligament Cells: Y. Hosokawa, et al.; Inflammation
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Calcitonin gene-related peptide is a key factor in the homing of transplanted human MSCs to sites of spinal cord injury: Y. Zhang, et al.; Sci. Rep.
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Full Text
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Full Text
Hypotonic Stress-Induced Down-Regulation of Claudin-1 and -2 Mediated by Dephosphorylation and Clathrin-Dependent Endocytosis in Renal Tubular Epithelial Cells: N. Fujii, et al.; J. Biol. Chem.
291, 24787 (2016),
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Full Text
NORE1A induction by membrane-bound CD40L (mCD40L) contributes to CD40L-induced cell death and G1 growth arrest in p21-mediated mechanism: T. Elmetwali, et al.; Cell Death Dis.
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Tunicamycin-induced Endoplasmic Reticulum Stress Upregulates the Expression of Pentraxin 3 in Human Retinal Pigment Epithelial Cells: N. Hwang, et al.; Korean J. Ophthalmol.
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Amphiregulin may be a new biomarker of classically activated macrophages: C. Meng, et al.; Biochem. Biophys. Res. Commun.
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Andrographolide decreased VEGFD expression in hepatoma cancer cells by inducing ubiquitin/proteasome-mediated cFos protein degradation: L. Ji, et al.; Biochim. Biophys. Acta.
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CArG-driven GADD45α activated by resveratrol inhibits lung cancer cells: Q. Shi, et al.; Genes Cancer
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Glycated albumin triggers fibrosis and apoptosis via an NADPH oxidase/Nox4-MAPK pathway-dependent mechanism in renal proximal tubular cells: W. Qi, et al.; Mol. Cell. Endocrinol.
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Involvement of JNK and Caspase Activation in Hoiamide A-Induced Neurotoxicity in Neocortical Neurons: Z. Cao, et al.; Mar. Drugs
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Full Text
Involvement of p38 MAPK activation mediated through AT1 receptors on spinal astrocytes and neurons in angiotensin II- and III-induced nociceptive behavior in mice: W. Nemoto, et al.; Neuropharmacology
99, 221 (2015),
Application(s): Injection into mice,
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Phloretin ameliorates chemokines and ICAM-1 expression via blocking of the NF-κB pathway in the TNF-α-induced HaCaT human keratinocytes: W.C. Huang, et al.; Int. Immunopharmacol.
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Suppression of death receptor 5 enhances cancer cell invasion and metastasis through activation of caspase-8/TRAF2-mediated signaling: Y.T. Oh, et al.; Oncotarget
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Full Text
PP2A inhibitors suppress migration and growth of PANC-1 pancreatic cancer cells through inhibition on the Wnt/β-catenin pathway by phosphorylation and degradation of β-catenin: M.Y. Wu, et al.; Oncol Rep.
32, 513 (2014),
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Full Text
Involvement of the RNA-binding protein ARE/poly(U)-binding factor 1 (AUF1) in the cytotoxic effects of proinflammatory cytokines on pancreatic beta cells: E. Roggli, et al.; Diabetologia
55, 1699 (2012),
Application(s): Treatment of pancreatic beta cells,
Abstract;
CpG Protects Human Monocytic Cells against HIV-Vpr-Induced Apoptosis by Cellular Inhibitor of Apoptosis-2 through the Calcium-Activated JNK Pathway in a TLR9-Independent Manner: M. Saxena, et al.; J. Immunol.
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Full Text
Mechanism and Repertoire of ASC-Mediated Gene Expression: M. Hasegawa, et al.; J. Immunol.
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Inhibition of c-Jun NH2-terminal kinase activity improves ischemia/reperfusion injury in rat lungs: M. Ishii, et al.; J. Immunol.
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An inhibitor of c-jun aminoterminal kinase (SP600125) represses c-Jun activation, DNA-binding and PMA-inducible 92-kDa type IV collagenase expression: M. Shin, et al.; Biochim. Biophys. Acta
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c-Jun N-terminal kinase is required for metalloproteinase expression and joint destruction in inflammatory arthritis: Z. Han, et al.; J. Clin. Invest.
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Full Text
Role of JNK in hypertonic activation of Cl(-)-dependent Na(+)/H(+) exchange in Xenopus oocytes: G.G. Goss, et al.; Am. J. Physiol. Cell. Physiol.
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SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase: B.L. Bennett, et al.; PNAS
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Full Text
TAK1/JNK and p38 have opposite effects on rat hepatic stellate cells: B. Schnabl, et al.; Hepatology
34, 953 (2001),
Abstract;
