Replaces Prod. #: ALX-350-045
Marine sesterterpenoid. Irreversibly inhibits human synovial phospholipase A2 (PLA2, IC50=0.02-0.2µM depending on substrate) as well as bee (IC50=0.05µM) and cobra venom (IC50=2µM) PLA2 by covalently modifying lysine residues. Inhibits human recombinant sPLA2 (IC50=4.7µM) selectively over cPLA2. Also inhibits phospholipase C (PLC, IC50=1.5µM) and Ca2+ channels. Inhibits superoxide generation in polymorphonuclear leukocytes. Inhibits the release of arachidonate in cultured mouse peritoneal macrophages and in a mouse in vivo model. Has anti-inflammatory and analgesic properties. Shows strong quorum sensing antagonism.
Product Details
| Formula: | C25H36O5 |
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| MW: | 416.6 |
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| CAS: | 75088-80-1 |
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| Purity: | ≥98% (TLC) |
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| Appearance: | Colorless to pale waxy or filmy residue. |
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| Solubility: | Soluble in DMSO (25mg/ml), 100% ethanol (25mg/ml) or methanol. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. Stock solutions are stable for up to 3 months at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Application(s): Screening on Juvenile and adult zebrafish,
Abstract;
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Abstract;
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Abstract;
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45, 218 (1996),
Abstract;
Identification of two specific lysines responsible for the inhibition of phospholipase A2 by manoalide: I.D. Bianco, et al.; Biochim. Biophys. Acta
1250, 197 (1995),
Abstract;
Phospholipase A2 activation in cultured mouse hepatocytes exposed to tumor necrosis factor-alpha: G.M. Adamson, et al.; J. Biochem. Toxicol.
9, 181 (1994),
Abstract;
Modulation of superoxide anion generation by manoalide, arachidonic acid and staurosporine in liver infiltrated neutrophils in a rat model of endotoxemia: A.M. Mayer & J.A. Spitzer; J. Pharmacol. Exp. Ther.
267, 400 (1993),
Abstract;
Secretory phospholipase A2 inhibitors and calmodulin antagonists as inhibitors of cytosolic phospholipase A2: W. C. Hope, et al.; Agents Actions
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Abstract;
Inhibition of venom phospholipases A2 by manoalide and manoalogue. Stoichiometry of incorporation: L.J. Reynolds, et al.; J. Biol. Chem.
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Abstract;
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Inactivation of human synovial fluid phospholipase A2 by the marine natural product, manoalide: P.B. Jacobson, et al.; Biochem. Pharmacol.
39, 1557 (1990),
Abstract;
Regulation of eicosanoid biosynthesis in vitro and in vivo by the marine natural product manoalide: a potent inactivator of venom phospholipases: A.M. Mayer, et al.; J. Pharmacol. Exp. Ther.
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Abstract;
Inactivation of bee venom phospholipase A2 by manoalide. A model based on the reactivity of manoalide with amino acids and peptide sequences: K.B. Glaser & R.S. Jacobs; Biochem. Pharmacol.
36, 2079 (1987),
Abstract;
Manoalide, a natural sesterterpenoid that inhibits calcium channels: L.A. Wheeler, et al.; J. Biol. Chem.
262, 6531 (1987),
Abstract;
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Cobra venom phospholipase A2 inhibition by manoalide. A novel type of phospholipase inhibitor: D. Lombardo & E.A. Dennis; J. Biol. Chem.
260, 7234 (1985),
Abstract;
