This fluorogenic peptide is often the substrate of choice for assaying the trypsin-like activity of purified proteasomes. Benefits include a low Km, high specific activity, and the fact that the substrate is cleaved exclusively at the R-AMC bond.
Product Details
| Alternative Name: | Ac-RLR-AMC |
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| Sequence: | Ac-Arg-Leu-Arg-AMC |
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| Formula: | C30H46N10O6 |
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| MW: | 642.7 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥97% (HPLC) |
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| Appearance: | Solid. |
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| Solubility: | Soluble in DMSO, methanol or aqueous solutions. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Several substrates with R-AMC scissile bonds can be used for assaying trypsin-like activity. One such substrate is Ac-Arg-Leu-Arg-AMC, which benefits from a low Km, high specific activity and the fact that it is cleaved exclusively at the R-AMC bond. This fluorogenic peptide is often the substrate of choice for assaying the trypsin-like activity of purified proteasomes. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A Transgenic Mouse Model of Eccentric Left Ventricular Hypertrophy With Preserved Ejection Fraction Exhibits Alterations in the Autophagy-Lysosomal Pathway: K. Wenzel, et al.; Front. Physiol.
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Abstract;
Targeting AML dependency on VCP-mediated DNA repair through a selective second-generation small molecule inhibitor: B. Roux, et al.; Sci. Transl. Med.
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Abstract;
The Architecture of the Anbu Complex Reflects an Evolutionary Intermediate at the Origin of the Proteasome System: A.C.D. Fuchs, et al.; Structure
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Full Text
Cardiac proteasome functional insufficiency plays a pathogenic role in diabetic cardiomyopathy: J. Li, et al.; J. Mol. Cell. Cardiol.
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Abstract;
Influence of the APOE genotype on hepatic stress response: Studies in APOE targeted replacement mice and human liver cells: J. Dose, et al.; Free Radic. Biol. Med.
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Application(s): Hepatic proteasome activity,
Abstract;
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine
2, 642 (2015),
Application(s): Fluorescence,
Abstract;
BAG3 induces the sequestration of proteasomal clients into cytoplasmic puncta: implications for a proteasome-to-autophagy switch: M. Minoia, et al.; Autophagy
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Abstract;
Full Text
The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget
5, 6252 (2014),
Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay,
Abstract;
Full Text
Importance of the proteosome's different proteolytic sites and the efficacy of inhibitors varies with the protein substrate.: A.F. Kisselev, et al.; J. Biol. Chem.
281, 8582 (2006),
Abstract;
Monitoring activity and inhibition of 26S proteasomes with fluorogenic peptide substrates: A.F. Kisselev & A.L. Goldberg; Methods Enzymol.
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Abstract;
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