Replaces Prod. #: ALX-380-283
Fluorescent, intercalating DNA-binding agent useful for identifying apoptotic cells by flow cytometry. Capable of identifying populations representing live, apoptotic, and late apoptotic/dead cells in a quick, simple, reproducible, cost-effective fashion. Inhibitor of DNA-primed RNA polymerase and DNA polymerase. Cytochemical probe. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. has antibacterial properties.
Product Details
| Alternative Name: | 7-AAD, 7AAD |
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| Formula: | C62H87N13O16 |
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| MW: | 1270.4 |
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| Source: | Semisynthetic from actinomycin D (Prod. No. BML-GR300-0005) |
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| CAS: | 7240-37-1 |
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| Purity: | ≥97% (HPLC) |
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| Appearance: | Purple crystalline solid. |
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| Solubility: | Soluble in DMSO (20mg/ml), methanol or 100% ethanol. Slightly soluble in water. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. |
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| Handling: | Protect from light. Hygroscopic. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Fascin1 Suppresses RIG-I-like Receptor Signaling and Interferon-β Production by Associating with IκB Kinase ϵ (IKK ϵ) in Colon Cancer: T. Matsumura, et al.; J. Biol. Chem.
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Abstract;
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Salmonella Activation of STAT3 Signaling by SarA Effector Promotes Intracellular Replication and Production of IL-10: S.L. Jaslow, et al.; Cell Rep.
23, 3525 (2018),
Abstract;
Cellular-FLIP, Raji isoform (c-FLIP R) modulates cell death induction upon T-cell activation and infection: T. Telieps, et al.; Eur. J. Immunol.
43, 1499 (2013),
Abstract;
Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D: I. Schmid, et al.; Nat. Protoc.
2, 187 (2007),
Abstract;
Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY: K. Toba, et al.; Eur. J. Haematol.
64, 10 (2000),
Abstract;
Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods: H. Lecoeur; J. Immunol. Methods
209, 111 (1997),
Abstract;
Hematopoietic cell protein-tyrosine phosphatase-deficient motheaten mice exhibit T cell apoptosis defect: X. Su, et al.; J. Immunol.
156, 4198 (1996),
Abstract;
The use of 7-amino actinomycin D in identifying apoptosis: simplicity of use and broad spectrum of application compared with other techniques: N.J. Philpott, et al.; Blood
87, 2244 (1996),
Abstract;
Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y: K. Toba, et al.; J. Immunol. Methods
182, 193 (1995),
Abstract;
A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry: I. Schmid, et al.; J. Immunol. Methods
170, 145 (1994),
Abstract;
Flow cytometric immunofluorescence assay for quantification of cyclobutyldithymine dimers in separate phases of the cell cycle: R.J. Berg, et al.; Carcinogenesis
14, 103 (1993),
Abstract;
Binding specificities of actinomycin D to non-self-complementary -XGCY- tetranucleotide sequences: F.M. Chen; Biochemistry
31, 6223 (1992),
Abstract;
Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry: I. Schmid, et al.; Cytometry
13, 204 (1992),
Abstract;
Improved staining method for the simultaneous flow cytofluorometric analysis of DNA content, S-phase fraction, and surface phenotype using single laser instrumentation: K. Toba, et al.; Cytometry
13, 60 (1992),
Abstract;
Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-amino-actinomycin D and single laser excitation: applications to study of cell activation and the cell cycle of murine Ly-1 B cells: P.S. Rabinovitch, et al.; J. Immunol.
136, 2769 (1986),
Abstract;
7-Amino-actinomycin D as a cytochemical probe. I. Spectral properties: J.E. Gill, et al.; J. Histochem. Cytochem.
23, 793 (1975),
Abstract;
7-substituted actinomycin D analogs. Chemical and growth-inhibitory studies: S.K. Sengupta, et al.; J. Med. Chem.
18, 1175 (1975),
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