Replaces Prod. #: ALX-350-281
Cardiotonic steroid specific Na+/K+-ATPase inhibitor. More potent than ouabain (Prod. No. BML-CM109). Induces cell differentiation. Induces apoptosis in U937 and HL-60 cells by activation of AP-1 via a MAPK pathway that involves JNK. Regulates Tiam1 gene. Down regulates expression of 14-3-3 protein.
Product Details
| Alternative Name: | (3β,5β)-3,14-Dihydroxybufa-20,22-dienolide, 5β,20(22)-Bufadienolide-3β,14-diol |
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| Formula: | C24H34O4 |
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| MW: | 386.5 |
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| CAS: | 465-21-4 |
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| MI: | 14: 1471 |
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| RTECS: | EI2962500 |
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| Purity: | ≥97% (HPLC) |
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| Appearance: | White to off-white solid. |
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| Solubility: | Soluble in DMSO (25 mg/ml) or 100% ethanol (25 mg/ml, warm). |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 1 year after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 3 months at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cardiotonic steroids differentially affect intracellular Na+ and [Na+]i/[K+]i-independent signaling in C7-MDCK cells: O.A. Akimova, et al.; J. Biol. Chem.
280, 832 (2005),
Abstract;
Simultaneous determination of four bufadienolides in human liver by high-performance liquid chromatography: Z. Wang, et al.; Biomed. Chromatogr.
18, 318 (2004),
Abstract;
The T-cell suppressive effect of bufadienolides: structural requirements for their immunoregulatory activity: P. Terness, et al.; Int. Immunopharmacol.
1, 19 (2001),
Abstract;
Na+, K+-ATPase inhibitors down-regulate gene expression of the intracellular signaling protein 14-3-3 in rat lens: M.H. McGowan, et al.; J. Pharmacol. Exp. Ther.
289, 1559 (1999),
Abstract;
Tiam1 is involved in the regulation of bufalin-induced apoptosis in human leukemia cells: N. Kawazoe, et al.; Oncogene
18, 2413 (1999),
Abstract;
Activation of AP-1 is required for bufalin-induced apoptosis in human leukemia U937 cells: M. Watabe, et al.; Oncogene
16, 779 (1998),
Abstract;
Bufadienolides from animal and plant sources: L. Krenn & B. Kopp; Phytochemistry
48, 1 (1998), (Review),
Abstract;
Enhancement by bufalin of retinoic acid-induced differentiation of acute promyelocytic leukemia cells in primary culture: K. Yamada, et al.; Leuk. Res.
22, 589 (1998),
Abstract;
Neutralization of cardiac toxins oleandrin, oleandrigenin, bufalin, and cinobufotalin by digibind: monitoring the effect by measuring free digitoxin concentrations: A. Dasgupta & L. Emerson; Life Sci.
63, 781 (1998),
Abstract;
Actions of bufalin and cinobufotalin, two bufadienolides respectively more active and less active than ouabain, on ouabain binding and 86Rb uptake by human erythrocytes: A.A. Brownlee, et al.; Clin. Sci. (Lond.)
78, 169 (1990),
Abstract;
