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ATPase inhibitor
BML-AP303-0010 10 mg 131.00 USD
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Replaces Prod. #: ALX-350-281

Cardiotonic steroid specific Na+/K+-ATPase inhibitor. More potent than ouabain (Prod. No. BML-CM109). Induces cell differentiation. Induces apoptosis in U937 and HL-60 cells by activation of AP-1 via a MAPK pathway that involves JNK. Regulates Tiam1 gene. Down regulates expression of 14-3-3 protein.

Product Details

Alternative Name:(3β,5β)-3,14-Dihydroxybufa-20,22-dienolide, 5β,20(22)-Bufadienolide-3β,14-diol
MI:14: 1471
Purity:≥97% (HPLC)
Appearance:White to off-white solid.
Solubility:Soluble in DMSO (25 mg/ml) or 100% ethanol (25 mg/ml, warm).
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:Stable for at least 1 year after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 3 months at -20°C.
Regulatory Status:RUO - Research Use Only
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Product Literature References

Cardiotonic steroids differentially affect intracellular Na+ and [Na+]i/[K+]i-independent signaling in C7-MDCK cells: O.A. Akimova, et al.; J. Biol. Chem. 280, 832 (2005), Abstract;
Simultaneous determination of four bufadienolides in human liver by high-performance liquid chromatography: Z. Wang, et al.; Biomed. Chromatogr. 18, 318 (2004), Abstract;
The T-cell suppressive effect of bufadienolides: structural requirements for their immunoregulatory activity: P. Terness, et al.; Int. Immunopharmacol. 1, 19 (2001), Abstract;
Na+, K+-ATPase inhibitors down-regulate gene expression of the intracellular signaling protein 14-3-3 in rat lens: M.H. McGowan, et al.; J. Pharmacol. Exp. Ther. 289, 1559 (1999), Abstract;
Tiam1 is involved in the regulation of bufalin-induced apoptosis in human leukemia cells: N. Kawazoe, et al.; Oncogene 18, 2413 (1999), Abstract;
Activation of AP-1 is required for bufalin-induced apoptosis in human leukemia U937 cells: M. Watabe, et al.; Oncogene 16, 779 (1998), Abstract;
Bufadienolides from animal and plant sources: L. Krenn & B. Kopp; Phytochemistry 48, 1 (1998), (Review), Abstract;
Enhancement by bufalin of retinoic acid-induced differentiation of acute promyelocytic leukemia cells in primary culture: K. Yamada, et al.; Leuk. Res. 22, 589 (1998), Abstract;
Neutralization of cardiac toxins oleandrin, oleandrigenin, bufalin, and cinobufotalin by digibind: monitoring the effect by measuring free digitoxin concentrations: A. Dasgupta & L. Emerson; Life Sci. 63, 781 (1998), Abstract;
Actions of bufalin and cinobufotalin, two bufadienolides respectively more active and less active than ouabain, on ouabain binding and 86Rb uptake by human erythrocytes: A.A. Brownlee, et al.; Clin. Sci. (Lond.) 78, 169 (1990), Abstract;

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