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Proteasome 20S assay kit

BML-AK740-0001 96 wells 708.00 USD
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Replaces Prod. #: ALX-850-230

A QUANTIZYME® Assay System. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome. The kit includes purified, human erythrocyte 20S proteasome (20µg). The Suc-LLVY-AMC fluorogenic peptide substrate supplied with this kit is a proteasome substrate commonly used for measuring chymotrypsin-like peptidase activity. The detection of proteolytic activity is based on the release of free AMC fluorophore and offers the following advantages: non-radioactive, convenient real-time kinetics of substrate cleavage, and excellent sensitivity. A proteasome inhibitor, epoxomicin, is included as a test inhibitor for screening purposes.
Proteasome 20S assay kit Fig4A
Proteasome 20S assay kit Standard curve
Proteasome 20S assay kit Fig3A
Proteasome 20S assay kit Fig3B
Proteasome 20S assay kit Fig4B
Proteasome 20S assay kit time_courses
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Proteasome 20S assay kit Fig4A Proteasome 20S assay kit Standard curve Proteasome 20S assay kit Fig3A Proteasome 20S assay kit Fig3B Proteasome 20S assay kit Fig4B Proteasome 20S assay kit time_courses

Product Details

Applications:Fluorescent detection, HTS
Activity assay
Handling:Do not store at temperature below recommended storage condition.
Shipping:Blue Ice
Long Term Storage:-20°C
Contents:Proteasome 20S (human) (purified) (Prod. No. BML-PW8720)
Suc-Leu-Leu-Val-Tyr-AMC (Prod. No. BML-P802)
Proteasome Assay Buffer (Prod. No. BML-KI340)
Sodium Dodecyl Sulfate (Prod. No. BML-KI341)
Epoxomicin (Prod. No. BML-PI127)
AMC (7-Amino-4-methylcoumarin) Calibration Standard (Prod. No. BML-KI144)
1/2 Volume White Microplates (Prod. No. BML-KI571)
Regulatory Status:RUO - Research Use Only

Product Literature References

Invited review: Muscle protein breakdown and its assessment in periparturient dairy cows: H. Sadri, et al.; J. Dairy Sci. 106, 822 (2023), Abstract;
SUMOylation-mediated PSME3-20S proteasomal degradation of transcription factor CP2c is crucial for cell cycle progression: S.H. Son, et al.; Sci. Adv. 9, eadd4969 (2023), Abstract;
Pyrrolidine dithiocarbamate facilitates arsenic trioxide against pancreatic cancer via perturbing ubiquitin-proteasome pathway: S. Yu, et al.; Cancer Manag. Res. 12, 13149 (2020), Abstract; Full Text
Vitamin A Protects the Preterm Lamb Diaphragm Against Adverse Effects of Mechanical Ventilation: Y. Song, et al.; Front. Physiol. 9, 1119 (2018), Abstract; Full Text
Cycloheximide promotes paraptosis induced by inhibition of cyclophilins in glioblastoma multiforme: L. Wang, et al.; Cell. Death Dis. 8, e2807 (2017), Abstract; Full Text
Secondary Metabolites Produced by an Endophytic Fungus Pestalotiopsis sydowiana and Their 20S Proteasome Inhibitory Activities: X. Xia, et al.; Molecules 21, e944 (2016), Application(s): Proteasome inhibition assay, Abstract; Full Text
Interleukin-1 receptor antagonist protects against lipopolysaccharide induced diaphragm weakness in preterm lambs: K. Karisnan, et al.; PLoS One 10, e0124390 (2015), Abstract; Full Text
Novel green synthesis of gold nanoparticles using Citrullus lanatus rind and investigation of proteasome inhibitory activity, antibacterial, and antioxidant potential: J.K. Patra, et al.; Int. J. Nanomedicine 10, 7253 (2015), Application(s): Proteasome inhibitory potential of ANPs, Abstract; Full Text
Enhancing proteasome-lnhibitor effect by functionalized gold nanoparticles: S.C. Coelho, et al.; J. Biomed. Nanotechnol. 10, 717 (2014), Abstract;
Gold nanoparticle delivery-enhanced proteasome inhibitor effect in adenocarcinoma cells: S.C. Coelho, et al.; Expert Opin. Drug. Deliv. 10, 1345 (2013), Abstract;
The angiotensin converting enzyme inhibitor captopril protects nigrostriatal dopamine neurons in animal models of parkinsonism: P.K. Sonalla, et al.; Exp. Neurol. 250, 376 (2013), Abstract; Full Text
Angiotensin II induced catabolic effect and muscle atrophy are redox dependent: L.C. Semprun-Prieto, et al.; Biochem. Biophys. Res. Commun. 409, 217 (2011), Abstract; Full Text

General Literature References

Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity.: L. Meng et al.; PNAS 96, 10403 (1999), Abstract; Full Text
The proteasome, a novel protease regulated by multiple mechanisms.: G. N. DeMartino & C. A. Slaughter; J. Biol. Chem. 274, 22123 (1999), Abstract; Full Text
Kinetic studies of the branched chain amino acid preferring peptidase activity of the 20S proteasome: development of a continuous assay and inhibition by tripeptide aldehydes and clasto-lactacystin beta-lactone: T. A. McCormack et al.; Biochemistry 37, 7792 (1998), Abstract;
Catalytic properties of 26 S and 20 S proteasomes and radiolabeling of MB1, LMP7, and C7 subunits associated with trypsin-like and chymotrypsin-like activities.: J. Reidlinger et al.; J. Biol. Chem. 272, 24899 (1997), Abstract; Full Text
Kinetic characterization of the chymotryptic activity of the 20S proteasome.: R. L. Stein et al.; Biochemistry 35, 3899 (1996), Abstract;
Multicatalytic endopeptidase complex: proteasome.: A. J. Rivett et al.; Methods Enzymol. 244, 331 (1994), Abstract;

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