Replaces Prod. #: ALX-850-230
A QUANTIZYME® Assay System. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome. The kit includes purified, human erythrocyte 20S proteasome (20µg). The Suc-LLVY-AMC fluorogenic peptide substrate supplied with this kit is a proteasome substrate commonly used for measuring chymotrypsin-like peptidase activity. The detection of proteolytic activity is based on the release of free AMC fluorophore and offers the following advantages: non-radioactive, convenient real-time kinetics of substrate cleavage, and excellent sensitivity. A proteasome inhibitor, epoxomicin, is included as a test inhibitor for screening purposes.
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Product Details
| Applications: | Fluorescent detection, HTS
Activity assay
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| Handling: | Do not store at temperature below recommended storage condition. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | Proteasome 20S (human) (purified) (Prod. No. BML-PW8720)
Suc-Leu-Leu-Val-Tyr-AMC (Prod. No. BML-P802)
Proteasome Assay Buffer (Prod. No. BML-KI340)
Sodium Dodecyl Sulfate (Prod. No. BML-KI341)
Epoxomicin (Prod. No. BML-PI127)
AMC (7-Amino-4-methylcoumarin) Calibration Standard (Prod. No. BML-KI144)
1/2 Volume White Microplates (Prod. No. BML-KI571) |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Invited review: Muscle protein breakdown and its assessment in periparturient dairy cows: H. Sadri, et al.; J. Dairy Sci.
106, 822 (2023),
Abstract;
SUMOylation-mediated PSME3-20S proteasomal degradation of transcription factor CP2c is crucial for cell cycle progression: S.H. Son, et al.; Sci. Adv.
9, eadd4969 (2023),
Abstract;
Pyrrolidine dithiocarbamate facilitates arsenic trioxide against pancreatic cancer via perturbing ubiquitin-proteasome pathway: S. Yu, et al.; Cancer Manag. Res.
12, 13149 (2020),
Abstract;
Full Text
Vitamin A Protects the Preterm Lamb Diaphragm Against Adverse Effects of Mechanical Ventilation: Y. Song, et al.; Front. Physiol.
9, 1119 (2018),
Abstract;
Full Text
Cycloheximide promotes paraptosis induced by inhibition of cyclophilins in glioblastoma multiforme: L. Wang, et al.; Cell. Death Dis.
8, e2807 (2017),
Abstract;
Full Text
Secondary Metabolites Produced by an Endophytic Fungus Pestalotiopsis sydowiana and Their 20S Proteasome Inhibitory Activities: X. Xia, et al.; Molecules
21, e944 (2016),
Application(s): Proteasome inhibition assay,
Abstract;
Full Text
Interleukin-1 receptor antagonist protects against lipopolysaccharide induced diaphragm weakness in preterm lambs: K. Karisnan, et al.; PLoS One
10, e0124390 (2015),
Abstract;
Full Text
Novel green synthesis of gold nanoparticles using Citrullus lanatus rind and investigation of proteasome inhibitory activity, antibacterial, and antioxidant potential: J.K. Patra, et al.; Int. J. Nanomedicine
10, 7253 (2015),
Application(s): Proteasome inhibitory potential of ANPs,
Abstract;
Full Text
Enhancing proteasome-lnhibitor effect by functionalized gold nanoparticles: S.C. Coelho, et al.; J. Biomed. Nanotechnol.
10, 717 (2014),
Abstract;
Gold nanoparticle delivery-enhanced proteasome inhibitor effect in adenocarcinoma cells: S.C. Coelho, et al.; Expert Opin. Drug. Deliv.
10, 1345 (2013),
Abstract;
The angiotensin converting enzyme inhibitor captopril protects nigrostriatal dopamine neurons in animal models of parkinsonism: P.K. Sonalla, et al.; Exp. Neurol.
250, 376 (2013),
Abstract;
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Angiotensin II induced catabolic effect and muscle atrophy are redox dependent: L.C. Semprun-Prieto, et al.; Biochem. Biophys. Res. Commun.
409, 217 (2011),
Abstract;
Full Text
General Literature References
Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity.: L. Meng et al.; PNAS
96, 10403 (1999),
Abstract;
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The proteasome, a novel protease regulated by multiple mechanisms.: G. N. DeMartino & C. A. Slaughter; J. Biol. Chem.
274, 22123 (1999),
Abstract;
Full Text
Kinetic studies of the branched chain amino acid preferring peptidase activity of the 20S proteasome: development of a continuous assay and inhibition by tripeptide aldehydes and clasto-lactacystin beta-lactone: T. A. McCormack et al.; Biochemistry
37, 7792 (1998),
Abstract;
Catalytic properties of 26 S and 20 S proteasomes and radiolabeling of MB1, LMP7, and C7 subunits associated with trypsin-like and chymotrypsin-like activities.: J. Reidlinger et al.; J. Biol. Chem.
272, 24899 (1997),
Abstract;
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Kinetic characterization of the chymotryptic activity of the 20S proteasome.: R. L. Stein et al.; Biochemistry
35, 3899 (1996),
Abstract;
Multicatalytic endopeptidase complex: proteasome.: A. J. Rivett et al.; Methods Enzymol.
244, 331 (1994),
Abstract;
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