NOW INCLUDES BOTH COLORIMETRIC AND FLUOROGENIC SUBSTRATES! This kit is a complete assay system to measure protease activity of caspase-8. Cleavage of a tetrapeptide substrate is monitored colorimetrically at 405 nm or fluorimetrically (Ex. 360 nm/Em. 460 nm). Assays are performed in a convenient 96-microwell plate format. These kits are useful for screening inhibitors of caspase-8. This kit is not intended for use in cellular extract assays.
Product Details
| Alternative Name: | FLICE, Mch5, MACH |
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| Applications: | Colorimetric detection, Fluorescence microscopy, HTS
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Contents: | Caspase-8 enzyme (human) (recombinant), Ac-IETD-pNA colorimetric substrate, Ac-IETD-AMC fluorimetric substrate, calibration standards, aldehyde inhibitor, assay buffer, 1/2-volume 96-microwell plate, and instruction booklet. |
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| Technical Info/Product Notes: | Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | Q14790 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood
117, 5953 (2011),
Abstract;
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