NOW INCLUDES BOTH COLORIMETRIC AND FLUOROGENIC SUBSTRATES! This kit is a complete assay system for measuring caspase-3 and caspase-3-like activity (DEVDase) in cellular extracts. It is performed in a convenient 96-well format with all reagents necessary for preparing cell extracts, measuring caspase activity and calibrating the assay. PLUS, Caspase-3 enzyme is included! The caspase-3, is useful as a positive control, for testing cellular extracts for endogenous inhibitors, and more.
Product Details
| Alternative Name: | CPP32, Yama, Apopain |
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| Applications: | Colorimetric detection, Fluorescent detection, HTS
Activity assay
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Contents: | Caspase-3 enzyme (human, recombinant) (Prod. No. BML-SE169-9090) (300U, (30µl); 10 u/µl in assy buffer. One U=1 pmol/min at 30°C) Storage: -70°C, avoid freeze/thaw cycles pNA Substrate (Ac-DEVD-pNA, MW=637) (Prod. No. BML-P412-9090) (1ml; 2mM (1.3 mg/ml) in assay buffer) Storage: -70°C pNA calibration standard (p-nitroaniline; MW=138) (Prod. No. BML-KI106) (1 ml, 50µM in assay buffer. A405nm=0.525) Storage: -70°C AMC Substrate (Ac-DEVD-AMC; MW=676) (Prod. No. BML-P411-9090) (1 ml; 0.3mM (0.20 mg/ml) in assay buffer Storage: -70°C AMC calibration standard (7-amino-4-methylcoumarin; MW=175)(Prod. No. BML-KI107) (1 ml; 30µM in assy buffer) Storage: -70°C Inhibitor (Ac-DEvD-CHO; MW=502) (Prod. No. BML-P410-9090) (50µl; 0.1mM (0.05 mg/ml) in DMSO) Storage: -70°C Assay Buffer (Prod. No. BML-KI-111) (20 ml; 50mM HEPES, pH 7.4, 100mM sodium chloride, 0.1% CHAPS, 10mM dithiothreitol, 1mmM ethylenediaminetetraacetic acid, 10% glycerol) Storage: -70°C Cell lysis buffer (50mM HEPES, pH 7.4, 0.1% CHAPS, 5mM dithiothreitol, 0.1mM ethylenediaminetetraacetic acid) (Prod. No. BML-KI117) (30 ml; liquid in white screw-cap plastic bottle) Storage: -70°C ½-Volume microplate (Prod. No. BML-KI101) Storage: Room temperature |
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| Technical Info/Product Notes: | Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | P42574 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Irigenin reduces the expression of caspase-3 and matrix metalloproteinases, thus suppressing apoptosis and extracellular matrix degradation in TNF-α-stimulated nucleus pulposus cells: G. Zhang, et al.; Chem. Biol. Interact.
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Relationship between blastocoel cell-free DNA and day-5 blastocyst morphology: K. Rule, et al.; J. Assist. Reprod. Genet.
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Autophagy in premature senescent cells is activated via AMPK pathway: L. Guo, et al.; Int. J. Mol. Sci.
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Full Text
Wedelolactone, a medicinal plant-derived coumestan, induces caspase-dependent apoptosis in prostate cancer cells via downregulation of PKCε without inhibiting Akt: S. Sarveswaran, et al.; Int. J. Oncol.
41, 2191 (2012),
Application(s): Caspase-3 activity measured in human prostate cancer cell (LNCaP) lysate,
Abstract;
Full Text
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood
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Effect of ubiquitin carboxy‐terminal hydrolase 37 on apoptotic in A549 cells: Z. Chen, et al.; Cell Biochem. Funct.
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Abstract;
Apaf-1-independent programmed cell death in mouse development: A. Nagasaka, et al.; Cell Death Differ.
17, 931 (2010),
Application(s): Caspase-3 measurement in cell lysates and culture supernatants,
Abstract;
Full Text
The β2-adrenoceptor agonist clenbuterol elicits neuroprotective, anti-inflammatory and neurotrophic actions in the kainic acid model of excitotoxicity: L.C. Gleeson, et al.; Brain Behav. Immun.
24, 1354 (2010),
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General Literature References
Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis: S. Kothakota et al.; Science
278, 294 (1997),
Abstract;
Multiple species of CPP32 and Mch2 are the major active caspases present in apoptotic cells: L. Faleiro et al.; EMBO J.
16, 2271 (1997),
Abstract;
CPP32/apopain is a key interleukin 1 beta converting enzyme-like protease involved in Fas-mediated apoptosis: J. Schlegel et al.; J. Biol. Chem.
271, 1841 (1996),
Abstract;
ICE/CED3-like proteases as therapeutic targets for the control of inappropriate apoptosis: D. W. Nicholson et al.; Nat. Biotechnol.
14, 297 (1996),
Abstract;
Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases: S. M. Srinivasula et al.; PNAS
93, 14486 (1996),
Abstract;
Activation of the apoptotic protease CPP32 by cytotoxic T-cell-derived granzyme B: A. J. Darmon et al.; Nature
377, 446 (1995),
Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D. W. Nicholson et al.; Nature
376, 37 (1995),
Abstract;
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik et al.; PNAS
92, 9042 (1995),
Abstract;
Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase: M. Tewari et al.; Cell
81, 801 (1995),
Abstract;
CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme: T Fernandes-Alnemri et al.; J. Biol. Chem.
269, 30761 (1994),
Abstract;
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