NOW INCLUDES BOTH CHROMOGENIC AND FLUOROGENIC SUBSTRATES! This kit is a complete assay system to measure protease activity of caspase-3. Cleavage of a tetrapeptide substrate is monitored colorimetrically by increased absorption at 405 nm or fluorimetrically (Ex. 360 nm/Em. 460 nm). Assays are performed in a convenient 96-well plate format. This kit is useful for screening inhibitors of caspase-3, a therapeutic target. For assays of caspase-3 in cellular extracts, please see the CASPASE-3 Cellular Activity Assay Kit PLUS (Prod. No. BML-AK703).
Product Details
| Alternative Name: | CPP32, Yama, Apopain |
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| Applications: | Colorimetric detection, Fluorescent detection, HTS
Activity assay
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Contents: | Caspase-3 enzyme (human) (recombinant), Ac-DEVD-pNA colorimetric substrate, Ac-DEVD-AMC fluorimetric substrate, calibration standards, aldehyde inhibitor, assay buffer, 1/2-volume 96-microwell plate, and instruction booklet. |
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| Technical Info/Product Notes: | Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | P42574 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
miR‑3613‑3p/MAP3K2/p38/caspase‑3 pathway regulates the heat‑stress‑induced apoptosis of endothelial cells: J. Liu, et al.; Mol. Med. Rep.
24, 633 (2021),
Abstract;
The effects of hsa_circ_0000517/miR-326 axis on the progression of breast cancer cells and the prediction of miR-326 downstream targets in breast cancer: J. Yu, et al.; Pathol. Res. Pract.
227, 153638 (2021),
Abstract;
Defects at the Posttranscriptional Level Account for the Low TCRζ Chain Expression Detected in Gastric Cancer Independently of Caspase-3 Activity: A. Aguinaga-Barrilero, et al.; J. Immunol. Res.
2020, 1039458 (2020),
Abstract;
Full Text
The Ayurvedic plant Bacopa monnieri inhibits inflammatory pathways in the brain: M.D. Nemetchek, et al.; J. Ethnopharmacol.
8741, 30495 (2016),
Application(s): Enzyme inhibition assays; bacopa extracts,
Abstract;
Connective tissue growth factor induces apoptosis via caspase 3 in cultured human aortic smooth muscle cells: K. Hishikawa, et al.; Eur. J. Pharmacol.
392, 19 (2000),
Abstract;
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