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Cathepsin S drug discovery kit

BML-AK431-0001 96 wells 633.00 USD
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A QUANTIZYME® Assay System. The Cathepsin S Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin S. Using a convenient microplate format, cathepsin S activity is monitored fluorimetrically using the substrate provided in the kit.
Cathepsin S drug discovery kit Fig2
Kinetics of Z-VVR-AMC cleavage by Cathepsin S, 0.059mU/well; 37°C. Rates were obtained from the slope of the initial, linear portion of plots of AFU vs. time. Curve and kinetic parameters derived from a non-linear least squares fit to the Michaelis-Menten equation (Marquadt algorithm).
Cathepsin S drug discovery kit Fig1a
Example of inhibitor data. Using the control, the slope is equal to 34.28 RFU/sec. With the inhibitor (E-64), the slope is equal to 0 RFU/sec. The % activity remaining after using the inhibitor can be calculated as follow: (0/34.28) x 100 = 0%
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Cathepsin S drug discovery kit Fig2 Cathepsin S drug discovery kit Fig1a

Product Details

Applications:Fluorescent detection, HTS
Activity assay
Application Notes:Complete system designed to screen modulators of cathepsin S activity. The assays are performed in a convenient 96-well microplate format.
Use/Stability:The enzyme component should be thawed as close to the time of use as is possible, and held on ice while in use. The enzyme can be frozen and thawed up to 5 times, but care should be taken to minimize both the time the enzyme spends on ice, and the number of times it is frozen and thawed. Do not hold the diluted enzyme at assay temperature (e.g 37°C) for an extended time before starting the assay.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Shipping:Dry Ice
Long Term Storage:-80°C
Contents:Cathepsin S enzyme (human) (recombinant), AMC substrate, AMC calibration standard, inhibitor, assay buffer, 1/2-volume white microplate.
UniProt ID:P25774
Regulatory Status:RUO - Research Use Only

Product Literature References

Nitrated Fatty Acids Reverse Cigarette Smoke-Induced Alveolar Macrophage Activation and Inhibit Protease Activity via Electrophilic S-Alkylation: A.T. Reddy, et al.; PLoS One 11, e0153336 (2016), Application(s): Cat S enzyme inhibition assay, Abstract; Full Text

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