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Matrix Metalloproteinase-13 (MMP-13) fluorometric drug discovery kit

BML-AK413-0001 96 wells 561.00 USD
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A QUANTIZYME® Assay System. The MMP-13 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a quenched fluorogenic peptide: OMNIMMP® fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-13, a potential therapeutic target. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet.

Product Details

Alternative Name:Collagenase-3
Applications:Fluorescent detection, HTS
Activity assay
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Contents:MMP-13 enzyme (human) (recombinant), substrate, calibration standard, inhibitor, assay buffer, 96-well white microplate.
UniProt ID:P45452
Regulatory Status:RUO - Research Use Only

Product Literature References

Doxycycline and its quaternary ammonium derivative for adjuvant therapies of chondrosarcoma: I. Miladi, et al.; Cancer Chemother. Pharmacol. 80, 517 (2017), Abstract;
Conditional activation of β‐catenin signaling in mice leads to severe defects in intervertebral disc tissue: M. Wang, et al.; Arthritis Rheum. 64, 2611 (2012), Application(s): Inhibition assay, Abstract; Full Text

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