A QUANTIZYME® Assay System. The MMP-13 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a quenched fluorogenic peptide: OMNIMMP® fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-13, a potential therapeutic target. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet.
Product Details
| Alternative Name: | Collagenase-3 |
| |
| Applications: | Fluorescent detection, HTS
Activity assay
|
| |
| Shipping: | Dry Ice |
| |
| Long Term Storage: | -80°C |
| |
| Contents: | MMP-13 enzyme (human) (recombinant), substrate, calibration standard, inhibitor, assay buffer, 96-well white microplate. |
| |
| UniProt ID: | P45452 |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Doxycycline and its quaternary ammonium derivative for adjuvant therapies of chondrosarcoma: I. Miladi, et al.; Cancer Chemother. Pharmacol.
80, 517 (2017),
Abstract;
Conditional activation of β‐catenin signaling in mice leads to severe defects in intervertebral disc tissue: M. Wang, et al.; Arthritis Rheum.
64, 2611 (2012),
Application(s): Inhibition assay,
Abstract;
Full Text
