Elevated cathepsin enzyme activity often indicates the presence of a pathological condition including cancer, Alzheimer's disease, autoimmune disease, and osteoporosis. Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy. Once cells are grown, staining is completed in just a few minutes. Hoechst 33342 stain and Acridine Orange are also included to allow visualization of nuclei and lysosomes.
Product Details
| Applications: | Fluorescence microscopy, Fluorescent detection
|
| |
| Application Notes: | Cited sample types include mouse microglial cells, human monocytic leukemia cells, and human PBMC. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | +4°C |
| |
| Contents: | CV-(RR)2 reagent, Acridine Orange, Hoechst 33342 stain |
| |
| UniProt ID: | P07858 |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Dysfunctional autophagy following exposure to pro-inflammatory cytokines contributes to pancreatic β-cell apoptosis: M. Lambelet et al.; Cell Death Dis.
9, 96 (2018),
Abstract;
Full Text
Differential pathways for interleukin-1β production activated by chromogranin A and amyloid β in microglia: Z. Wu, et al.; Neurobiol. Aging
34, 2715 (2013),
Application(s): Activity detected in mouse microglial cells.,
Abstract;
Ethanol Inhibits Activation of NLRP3 and AIM2 Inflammasomes in Human Macrophages-A Novel Anti-Inflammatory Action of Alcohol: K. Nurmi, et al.; PLoS One
8, e78537 (2013),
Application(s): Activity detected in human monocytic leukemia cells.,
Abstract;
Full Text
Dysregulation of macrophage-secreted cathepsin B contributes to HIV-1-linked neuronal apoptosis: E.J. Rodriguez-Franco, et al.; PLoS One
7, e3657 (2012),
Application(s): Activity detected in human PBMC.,
Abstract;
Full Text
Microglial cathepsin B contributes to the initiation of peripheral inflammation-induced chronic pain: L. Sun, et al.; J. Neurosci.
32, 11330 (2012),
Application(s): Activity detected in mouse microglial cells.,
Abstract;
Full Text
Serum amyloid A activates the NLRP3 inflammasome via P2X7 receptor and a cathepsin B-sensitive pathway: K. Niemi, et al.; J. Immunol.
186, 6119 (2011),
Application(s): Activity detected in human monocytic leukemia cells.,
Abstract;
Full Text
Cholesterol crystals activate the NLRP3 inflammasome in human macrophages: a novel link between cholesterol metabolism and inflammation: K. Rajamäki, et al.; PLoS One
5, e11765 (2010),
Application(s): Activity detected in human monocytic leukemia cells.,
Abstract;
Full Text
