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Carboxyfluorescein multi-caspase activity kit

BML-AK117-0001 1 Kit 856.00 USD
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The Carboxyfluorescein Multi-Caspase Activity Kit detects apoptosis through the covalent binding of a green fluorescent probe to active caspase enzymes in living cells. The carboxyfluorescein-labeled caspase inhibitor probe (FAM-VAD-FMK; excitation at 490 nm and emission at 520 nm), comprising a carboxyfluorescein derivative of valylalanylaspartic acid fluoromethyl ketone (VAD-FMK), covalently binds to the reactive cysteine (Cys 285) on the large subunit of active caspase heterodimers. This probe is both cell permeable and non-cytotoxic during the time of the labeling procedure and allows detection of apoptosis by fluorescence microscopy, flow cytometry, and 96-well-plate based fluorometry. Use of the fluorescence microscope provides a qualitative view of the level of apoptosis induced as compared to the use of a fluorometer or a flow cytometer which will generate quantitative data. The Sulforhodamine Multi-Caspase Activity Kit for Apoptosis Detection is a related kit that contains a sulforhodamine-labeled probe (Ex: 550, Em: 595).

Product Details

Applications:Fluorescent detection, HTS
Activity assay
Handling:Store the unopened kit (and each unopened component) at 2-8°C until the expiration date. Protect the FAM-VAD-FMK reagent from light at all times. Once reconstituted, the 150X FAM-VAD-FMK stock should be stored at -20°C protected from light. This reagent is stable for up to 6 months and may be thawed twice during that time.
Shipping:Blue Ice
Long Term Storage:+4°C
Contents:FAM-VAD-FMK Reagent, 10X Wash Buffer, Fixative, Propidium Iodide.
Regulatory Status:RUO - Research Use Only

Product Literature References

Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): Insect Larval Hemocytes, Abstract; Full Text
Octanoic Acid - An Insecticidal Metabolite of Conidiobolus coronatus (Entomopthorales) That Affects Two Majors Antifungal Protection Systems in Galleria mellonella (Lepidoptera): Cuticular Lipids and Hemocyte: A. Kaczmarek, et al.; Int. J. Mol. Sci. 23, 5204 (2022), Abstract;
Mcl-1 is a key regulator of the ovarian reserve: S. Omari, et al.; Cell. Death Dis. 6, e1755 (2015), Application(s): Assay using mouse oocytes, Abstract; Full Text

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