The Carboxyfluorescein Multi-Caspase Activity Kit detects apoptosis through the covalent binding of a green fluorescent probe to active caspase enzymes in living cells. The carboxyfluorescein-labeled caspase inhibitor probe (FAM-VAD-FMK; excitation at 490 nm and emission at 520 nm), comprising a carboxyfluorescein derivative of valylalanylaspartic acid fluoromethyl ketone (VAD-FMK), covalently binds to the reactive cysteine (Cys 285) on the large subunit of active caspase heterodimers. This probe is both cell permeable and non-cytotoxic during the time of the labeling procedure and allows detection of apoptosis by fluorescence microscopy, flow cytometry, and 96-well-plate based fluorometry. Use of the fluorescence microscope provides a qualitative view of the level of apoptosis induced as compared to the use of a fluorometer or a flow cytometer which will generate quantitative data. The Sulforhodamine Multi-Caspase Activity Kit for Apoptosis Detection is a related kit that contains a sulforhodamine-labeled probe (Ex: 550, Em: 595).
Product Details
Applications: | Fluorescent detection, HTS Activity assay
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Handling: | Store the unopened kit (and each unopened component) at 2-8°C until the expiration date. Protect the FAM-VAD-FMK reagent from light at all times. Once reconstituted, the 150X FAM-VAD-FMK stock should be stored at -20°C protected from light. This reagent is stable for up to 6 months and may be thawed twice during that time. |
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Shipping: | Blue Ice |
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Long Term Storage: | +4°C |
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Contents: | FAM-VAD-FMK Reagent, 10X Wash Buffer, Fixative, Propidium Iodide. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol.
12, 774086 (2022),
Application(s): Insect Larval Hemocytes,
Abstract;
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Octanoic Acid - An Insecticidal Metabolite of Conidiobolus coronatus (Entomopthorales) That Affects Two Majors Antifungal Protection Systems in Galleria mellonella (Lepidoptera): Cuticular Lipids and Hemocyte: A. Kaczmarek, et al.; Int. J. Mol. Sci.
23, 5204 (2022),
Abstract;
Mcl-1 is a key regulator of the ovarian reserve: S. Omari, et al.; Cell. Death Dis.
6, e1755 (2015),
Application(s): Assay using mouse oocytes,
Abstract;
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