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BIOMOL® Green

A simple and convenient method for colorimetric phosphate quantitation.
 
BML-AK111-0250 250 ml 140.00 USD
 
BML-AK111-1000 1 L 374.00 USD
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  • Convenient one-step reagent, no mixing needed
  • Extremely stable with a long shelf-life, >6 months at +4°C
  • Excellent sensitivity, ~50 pmol in 100 µl
  • Can be used in cuvette or microplate-based assays, making it ideal for high-throughput applications
  • Simple and complete kit that comes with a standardized phosphate solution for assay calibration
BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL® Green doesn’t require multiple solutions or reagents prepared fresh on the day of the assay. BIOMOL® Green is extremely stable (>6 mos. at 4°C) and is simply mixed, at room temperature, with any enzymatic reaction that has released free phosphate. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.BIOMOL® Green has perhaps been most widely applied to protein phosphatase assays. Typically, a protein phosphatase (e.g. PTP1B or LAR) is simply incubated with an appropriate phosphopeptide (e.g. IR5,9,10). Addition of BIOMOL® Green stops the reaction and begins color development, which is read 20-30 min. later. BIOMOL® Green may be used in cuvette or microplate-based assays and is ideal for high-throughput applications. Each kit comes with a standardized phosphate solution for assay calibration.

Product Specification

Applications:FUNC, Colorimetric detection, HTS
 
Application Notes:Mainly used in protein phosphatase assays. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Contents:Biomol® Green Reagent (Prod. No. BML-AK111) (1l or 250ml; liquid in screw cap plastic bottle) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects.
Phosphate Standard (Prod. No. BML-KI102) (1ml; 800µm phosphate in distilled water) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Crystal structure and catalytic activity of the PPM1K N94K mutant: M. Rostaminasab Dolatabad, et al.; J. Neurochem. 148, 550 (2019), Abstract;
De Novo Mutations Affecting the Catalytic Cα Subunit of PP2A, PPP2CA, Cause Syndromic Intellectual Disability Resembling Other PP2A-Related Neurodevelopmental Disorders: S. Reynhout, et al.; Am. J. Hum. Genet. 104, 139 (2019), Abstract; Full Text
Evaluation of binding and inhibition mechanism of dietary phytochemicals with sphingosine kinase 1: Towards targeted anticancer therapy: P. Gupta, et al.; Sci. Rep. 9, 18727 (2019), Abstract; Full Text
Investigation of inhibitory potential of quercetin to the pyruvate dehydrogenase kinase 3: Towards implications in anticancer therapy: R. Dahiya, et al.; Int. J. Biol. Macromol. 136, 1076 (2019), Abstract;
Lipoxin A4 ameliorates lipopolysaccharide-induced lung injury through stimulating epithelial proliferation, reducing epithelial cell apoptosis and inhibits epithelial–mesenchymal transition: J. Yang, et al.; Respir. Res. 20, 192 (2019), Abstract; Full Text
Probing the Inhibition of Microtubule Affinity Regulating Kinase 4 by N-Substituted Acridones: M. Voura, et al.; Sci. Rep. 9, 1676 (2019), Application(s): Enzyme Activity of MARK4 (Kinase inhibition assay), Abstract; Full Text
Trypanosoma cruzi Phosphomannomutase and Guanosine Diphosphate-Mannose Pyrophosphorylase Ligandability Assessment: F. Zmuda, et al.; Antimicrob. Agents Chemother. 63, e01082-19 (2019), Abstract; Full Text
A dynamic allosteric pathway underlies Rad50 ABC ATPase function in DNA repair: Z.K. Boswell, et al.; Sci Rep. 8, 1639 (2018), Abstract; Full Text
An essential Staphylococcus aureus cell division protein directly regulates FtsZ dynamics: P. Eswara, et al.; Elife 7, (2018), Abstract; Full Text
Coupling of polymerase and carrier lipid phosphatase prevents product inhibition in peptidoglycan synthesis: V.M. Hernadez-Rocamora, et al.; Cell Surf. 2, 1 (2018), Abstract; Full Text
HDX-MS reveals dysregulated checkpoints that compromise discrimination against self RNA during RIG-I mediated autoimmunity: J. Zheng, et al.; Nat. Commun. 9, 5366 (2018), Abstract; Full Text
Identification of a pyrophosphate-dependent kinase and its donor selectivity determinants: R. Nagata, et al.; Nat. Commun. 9, 1765 (2018), Abstract; Full Text
Investigation of Molecular Mechanism of Recognition between Citral and MARK4: A Newer Therapeutic Approach to Attenuate Cancer Cell Progression: F. Naz, et al.; Int. J. Biol. Macromol. 107(Pt B), 2580 (2018), Abstract;
Isoform-specific role of Na/K-ATPase α1 in skeletal muscle: L.C. Kutz, et al.; Am. J. Physiol. Endocrinol. Metab. 314, E620 (2018), Abstract;
K+ and Rb+ Affinities of the Na,K-ATPase a1 and a2 Isozymes: An Application of ICP-MS for Quantification of Na+ Pump Kinetics in Myofibers: H. Hakimjavadi, et al.; Int. J. Mol. Sci. 19, E2725 (2018), Abstract; Full Text
Phenethyl isothiocyanate activates leptin signaling and decreases food intake: M. Yagi, et al.; PLoS One 13, e0206748 (2018), Abstract;
Recognizability of heterologous co-chaperones with Streptococcus intermedius DnaK and Escherichia coli DnaK: T. Tomoyasu, et al.; Microbiol. Immunol. 62, 681 (2018), Abstract;
Chemical Validation of Methionyl-tRNA Synthetase as a Druggable Target in Leishmania donovani: L.S. Torrie, et al.; ACS Infect. Dis. 3(10), 718-727 (2017), Abstract;
Construction of an alkaline phosphatase-specific two-photon probe and its imaging application in living cells and tissues: H. Zhang, et al.; Biomaterials 140, 220 (2017), Abstract;
De Novo Missense Mutations in DHX30 Impair Global Translation and Cause a Neurodevelopmental Disorder: D. Lessel, et al.; Am. J. Hum. Genet. 101, 716 (2017), Abstract; Full Text
EYA1's conformation-specificity in dephosphorylating phosphothreonine in Myc and its activity on Myc stabilization in breast cancer: J. Li, et al.; Mol. Cell. Biol. 37, e00499-16 (2017), Abstract;
Pharmacological inhibition of protein tyrosine phosphatase 1B protects against atherosclerotic plaque formation in the LDLR-/- mouse model of atherosclerosis: D. Thompson, et al.; Clin. Sci. (Lond.) 131, 2489 (2017), Abstract;
G-rich telomeric and ribosomal DNA sequences from the fission yeast genome form stable G-quadruplex DNA structures in vitro and are unwound by the Pfh1 DNA helicase: M. Wallgren, et al.; Nucleic Acids Res. 44, 6213 (2016), Application(s): ATPase assay, Abstract; Full Text
Ischemia/reperfusion‐induced alterations of enzymatic and signaling functions of the rat cardiac Na+/K+‐ATPase: protection by ouabain preconditioning: A. Belliard, et al.; Physiol. Rep. 4, e12991 (2016), Application(s): Na+/K+‐ATPase activity, rat heart tissue homogenates, Abstract; Full Text
SERCA, complex I of the respiratory chain and ATP-synthase inhibition are involved in pleiotropic effects of NS1619 on endothelial cells: A. Lukasiak, et al. ; Eur. J. Pharmacol. 786, 137 (2016), Application(s): Ca2+-ATPase activity measurements, Abstract;
Structure and function of outer dynein arm intermediate and light chain complex: T. Oda, et al.; Mol. Biol. Cell 27, 1051 (2016), Abstract; Full Text
Zinc coordination is essential for the function and activity of the type II secretion ATPase EpsE: C.S. Rule, et al.; MicrobiologyOpen 5, 870 (2016), Application(s): ATPase activity assays, Abstract; Full Text
Altered cofactor regulation with disease-associated p97/VCP mutations: X. Zhang, et al.; PNAS 112, E1705 (2015), Application(s): Assay, Abstract; Full Text
Characterization of Wall Teichoic Acid Degradation by the Bacteriophage ϕ 29 Appendage Protein GP12 Using Synthetic Substrate Analogs: C.L. Myers, et al.; J. Biol. Chem. 290, 19133 (2015), Application(s): Measurement of inorganic phosphate, Abstract; Full Text
Covalent Docking Predicts Substrates for Haloalkanoate Dehalogenase Superfamily Phosphatases: N. London, et al.; Biochemistry 54, 528 (2015), Application(s): Empirical screen, Abstract; Full Text
Direct Ionic Regulation of the Activity of Myo-Inositol Biosynthesis Enzymes in Mozambique Tilapia: F.D. Villarreal, et al.; PLoS One 10, e0123212 (2015), Application(s): Colorimetric detection, Abstract; Full Text
Functional structure and physiological functions of mammalian wild-type HSP60: T. Okamoto, et al.; Arch. Biochem. Biophys. 586, 10 (2015), Application(s): ATPase activity assay, Abstract;
Membrane Topology and Biochemical Characterization of the Escherichia coli BacA Undecaprenyl-Pyrophosphate Phosphatase: G. Manat, et al. ; PLoS One 10, e0142870 (2015), Application(s): Phosphatase assay, Abstract; Full Text
Phospholemman is not required for the acute stimulation of Na+-K+-ATPase α2-activity during skeletal muscle fatigue: P. Manoharan, et al.; Am. J. Physiol. Cell Physiol. 309, C813 (2015), Abstract;
Rare Manifestation of a c.290 C>T, p.Gly97Glu VCP Mutation: N. U. Jerath, et al.; Case Rep. Genet. 2015, 239167 (2015), Application(s): Microplate, Abstract; Full Text
The catalytic role of the M2 metal ion in PP2Cα: C. Pan, et al.; Sci. Rep. 5, 8560 (2015), Abstract;
The second-sphere residue T263 is important for the function and catalytic activity of PTP1B via interaction with the WPD-loop: P. Xiao, et al.; Int. J. Biochem. Cell Biol. 57, 84 (2014), Abstract;
Protein phosphatase 4 is phosphorylated and inactivated by Cdk in response to spindle toxins and interacts with γ-tubulin: M. Voss, et al.; Cell Cycle 12, 2876 (2013), Application(s): Assay, Abstract; Full Text
Cdc14 phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine: S.C. Bremmer, et al.; J. Biol. Chem. 287, 1662 (2012), Application(s): Detection of inorganic phosphate, Abstract; Full Text
Osteoclast migration on phosphorylated osteopontin is regulated by endogenous tartrate-resistant acid phosphatase: B. Ek-Rylander, et al.; Exp. Cell Res. 316, 443 (2010), Abstract;
Reduction of hypothalamic protein tyrosine phosphatase improves insulin and leptin resistance in diet-induced obese rats: P.K. Picardi, et al.; Endocrinology 149, 3870 (2008), Abstract; Full Text
Big mitogen-activated protein kinase 1 (BMK1)/extracellular signal regulated kinase 5 (ERK5) is involved in platelet-derived growth factor (PDGF)-induced vascular smooth muscle cell migration: Y. Izawa, et al.; Hypertens. Res. 20, 1107 (2007), Abstract;
Enhanced sensitivity of insulin-resistant adipocytes to vanadate is associated with oxidative stress and decreased reduction of vanadate (+5) to vanadyl (+4): B. Lu, et al.; J. Biol. Chem. 276, 35589 (2001), Abstract;
Characterization and kinetic analysis of the intracellular domain of human protein tyrosine phosphatase beta (HPTP beta) using synthetic phosphopeptides: K. W. Harder et al.; Biochem. J. 298 , 395 (1994), Abstract;
Use of fluorinated tyrosine phosphates to probe the substrate specificity of the low molecular weight phosphatase activity of calcineurin: B. Martin et al.; J. Biol. Chem. 260, 14932 (1985), Abstract;

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