Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active recombinant HAT as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
Figure 1: Analysis of HAT Activity in HeLa Nuclear Extract. HeLa nuclear extract in various amounts was incubated with HAT substrate. Activity was analyzed in a microplate reader at 440nm according to the kit instructions.
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Product Details
| Alternative Name: | Histone acetyltransferase |
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| Applications: | Colorimetric detection
Activity assay
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Contents: | 2x HAT Assay Buffer
HAT Substrate I
HAT Substrate II
NADH Generating Enzyme
NE (Nuclear Extract 4 mg/ml)
HAT Reconstitution Buffer |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Application(s): Measurement of HAT activity in CPTH6-treated cells,
Abstract;
Quercetin inhibits TNF-induced NF-kappaB transcription factor recruitment to proinflammatory gene promoters in murine intestinal epithelial cells: P.A. Ruiz, et al.; J. Nutr.
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Abstract;
