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Microcystins (Adda specific) ELISA kit

Patented ELISA for rapid detection of microcystins and nodularins.
 
ALX-850-319-KI01 96 wells 761.00 USD
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  • Specific – antibody to Adda moiety specifically binds microcystin & nodularin toxins
  • Sensitive – measure as little as 0.1ng/ml toxin
  • Convenient – no pre-sample preparation required
  • Rapid – analyze 41 duplicate samples in <2.5 hours
Polyclonal antibody prepared against the Adda moiety binds to microcystins and nodularins, allowing the congener-independent determination of these toxins and many of its congeners, and does not cross-react with other non-related toxins or compounds. No pre-sample preparation required. Total time for measurement is less than 2.5 hours. Enables simultaneous measurement of multiple samples at reasonable costs.
Microcystins (Adda specific) ELISA kit FIG1
Cross-reactivity pattern against microcystins and nodularin congeners
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Microcystins (Adda specific) ELISA kit FIG1

Product Specification

Sensitivity:0.1ng/ml (range 0.15 to 5ng/ml)
 
Assay Time:2 hour 30 minutes
 
Applications:ELISA
 
Application Notes:For the quantitative and sensitive congener-independent detection of microcystins and nodularin in water samples.
 
Wavelength:450 nm
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.
 
Technical Info/Product Notes:Patented technology. U.S. Patent No. 6,967,240. Worldwide Patent PCT WO 01/18059 A2.
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal
 

Product Literature References

Microcystins in European Noble Crayfish Astacus astacus in Lake Steinsfjorden, a Planktothrix-Dominated Lake: I. A. Samdal, et al.; Toxins (Basel) 12, 298 (2020), Abstract; Full Text
Attack of Microcystis aeruginosa bloom on a Ceratophyllum submersum field: ecotoxicological measurements in real environment with real microcystin exposure: A.Z. Ujvarosi, et al.; Sci. Total Environ. 662, 735 (2019), Application(s): ELISA using water bloom, water, and plant samples, Abstract;
Impacts of hydrogen peroxide and copper sulfate on the control of Microcystis aeruginosa and MC-LR and the inhibition of MC-LR degrading bacterium: M.M. Kansole, et al.; Water 9, 255 (2017), Full Text
Microcystin-LR biodegradation by Bacillus sp.: reaction rates and possible genes involved in the degradation: M.M. Kansole, et al.; Water 8, 508 (2016), Full Text
Isolation of toxic marine cyanobacteria and detection of microcystins in Thermaikos Gulf in Central Macedonia in Greece: M. Kalaitzidou, et al.; HAICTA 2015 (Proceedings of the 7th International Conference on Information and Communication Technologies in Agriculture, Food and Environment) 832 (2015), Full Text
Effect of irrigation with lake water containing microcystins on microcystin content and growth of ryegrass, clover, rape, and lettuce: J.R. Crush, et al.; Environ. Toxicol. 23, 246 (2008), Abstract;
A review of analytical methods for assessing the public health risk from microcystin in the aquatic environment: P.R. Hawkins, et al.; J. Water Supply 54, 509 (2005),
Congener-independent immunoassay for microcystins and nodularins: W.J. Fischer, et al.; Environ. Sci. Technol. 35, 4849 (2001), Abstract;

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