Replaces Prod. #: ALX-850-303/1
The CD14, soluble (human) ELISA kit is a complete assay for the quantitative determination of soluble human CD14 concentration. This kit is to be used with serum, plasma and cell culture medium from human origin.
Product Details
Sensitivity: | Range: 5-50ng/ml |
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Assay Time: | 1.5 hours + 14+/- 1 minute |
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Application Notes: | For the quantitative determination of soluble mouse CD14 in serum, plasma and cell culture medium. |
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Wavelength: | 450 nm |
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Species reactivity: | Mouse
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Quantity: | 96 wells (~80 tests) |
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Handling: | For long term storage keep vials 3 and 4 at -20°C. Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Contents: | 1 precoated ELISA module
1 vial detecting antibody (POD-labelled polyclonal antibodies to mouse CD14)
1 vial mouse CD14 standard (recombinant mouse CD14, lyophilized)
1 vial reference serum (2.7 ± 0.5, lyophilized)
2 tablets PBS
1 vial sample dilution buffer
1 vial Tween 20
1 vial stopping solution (ready-to-use)
1 vial substrate solution (ready-to-use)
1 vial detecting antibody dilution buffer |
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UniProt ID: | P10810 |
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Regulatory Status: | RUO - Research Use Only |
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Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Soybean polar lipids differently impact adipose tissue inflammation and the endotoxin transporters LBP and sCD14 in flaxseed vs. palm oil-rich diets: M. Lecomte, et al.; J. Nutr. Biochem.
43, 116 (2017),
Application(s): ELISA using mouse plasma,
Abstract;
High-fat diet action on adiposity, inflammation, and insulin sensitivity depends on the control low-fat diet: N. Benoit, et al.; Nutr. Res.
33, 952 (2013),
Application(s): Levels of LBP in mouse plasma,
Abstract;
n-3 PUFA added to high-fat diets affect differently adiposity and inflammation when carried by phospholipids or triacylglycerols in mice: M. Awada, et al.; Nutr. Metab. (Lond.)
10, 23 (2013),
Application(s): Levels of LBP in mouse plasma,
Abstract;
Full Text
Oil composition of high fat diet affects differently metabolic inflammation in connection with endotoxin receptors in mice: F. Laugerette, et al.; Am. J. Physiol. Endocrinol. Metab.
302, E374 (2012),
Application(s): ELISA using mouse plasma,
Abstract;
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