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CD14, soluble (mouse) ELISA kit

 
ALX-850-303-KI01 96 wells 1,356.00 USD
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Replaces Prod. #: ALX-850-303/1

The CD14, soluble (human) ELISA kit is a complete assay for the quantitative determination of soluble human CD14 concentration. This kit is to be used with serum, plasma and cell culture medium from human origin.

Product Details

Sensitivity:Range: 5-50ng/ml
 
Assay Time:1.5 hours + 14+/- 1 minute
 
Application Notes:For the quantitative determination of soluble mouse CD14 in serum, plasma and cell culture medium.
 
Wavelength:450 nm
 
Species reactivity:Mouse
 
Quantity:96 wells (~80 tests)
 
Handling:For long term storage keep vials 3 and 4 at -20°C. Avoid freeze/thaw cycles.
 
Shipping:Blue Ice
 
Short Term Storage:+4°C
 
Contents:1 precoated ELISA module
1 vial detecting antibody (POD-labelled polyclonal antibodies to mouse CD14)
1 vial mouse CD14 standard (recombinant mouse CD14, lyophilized)
1 vial reference serum (2.7 ± 0.5, lyophilized)
2 tablets PBS
1 vial sample dilution buffer
1 vial Tween 20
1 vial stopping solution (ready-to-use)
1 vial substrate solution (ready-to-use)
1 vial detecting antibody dilution buffer
 
UniProt ID:P10810
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Soybean polar lipids differently impact adipose tissue inflammation and the endotoxin transporters LBP and sCD14 in flaxseed vs. palm oil-rich diets: M. Lecomte, et al.; J. Nutr. Biochem. 43, 116 (2017), Application(s): ELISA using mouse plasma, Abstract;
High-fat diet action on adiposity, inflammation, and insulin sensitivity depends on the control low-fat diet: N. Benoit, et al.; Nutr. Res. 33, 952 (2013), Application(s): Levels of LBP in mouse plasma, Abstract;
n-3 PUFA added to high-fat diets affect differently adiposity and inflammation when carried by phospholipids or triacylglycerols in mice: M. Awada, et al.; Nutr. Metab. (Lond.) 10, 23 (2013), Application(s): Levels of LBP in mouse plasma, Abstract; Full Text
Oil composition of high fat diet affects differently metabolic inflammation in connection with endotoxin receptors in mice: F. Laugerette, et al.; Am. J. Physiol. Endocrinol. Metab. 302, E374 (2012), Application(s): ELISA using mouse plasma, Abstract;

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