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Cytochrome c releasing apoptosis assay kit

 
ALX-850-277-KI01 1 Kit 769.00 USD
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The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol. Cytochrome c released from mitochondria into cytosol is determined by Western blot using the cytochrome c antibody provided in the kit. The procedure is simple, straightforward and requires no ultracentrifugation.

Product Details

Application Notes:For the detection of cytochrome c translocation from mitochondria into cytosol during apoptosis.
 
Quantity:100 tests.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Contents:10ml Mitochondria Extraction Buffer, 20ml 5X Cytosol Extraction Buffer, 110µl DTT (1M), 1 vial 500X Protease Inhibitor Cocktail, 0.5ml Cytochrome c Antibody (0.2mg/ml).
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

FTY720 inhibits tumor growth and enhances the tumor‐suppressive effect of topotecan in neuroblastoma by interfering with the sphingolipid signaling pathway: M. Li, et al.; Pediatr. Blood Cancer 60, 1418 (2013), Abstract; Full Text
Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9 mediated activation of the mitochondrial death pathway: J. Wang, et al.; Mol. Med. Rep. 8, 787 (2013), Abstract;

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