FITC-labelled recombinant human annexin V shows bright green fluorescence. SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence (Ex(max): 488nm; Em(max): 530nm). Apoptotic cells show green fluorescence, dead cells show a higher level of green fluorescence and live cells show little or no fluorescence. By using the FL1 channel in FC, other channels are free for multiple labelling experiments.
Product Details
| Applications: | Flow Cytometry, Fluorescence microscopy
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| Application Notes: | For the detection of apoptotic cells by flow cytometry. |
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| Handling: | Protect from light. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Contents: | Annexin V-FITC, SYTOX green dye and binding buffer.
An assay protocol is included. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Activators and stimulators of soluble guanylate cyclase counteract myofibroblast differentiation of prostatic and dermal stromal cells: C. Zenzmaier, et al. ; Exp. Cell Res.
338, 162 (2015),
Application(s): Stained human dermal cells to measure apoptosis,
Abstract;
