FITC-labelled recombinant human annexin V shows bright green fluorescence. SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence (Ex(max): 488nm; Em(max): 530nm). Apoptotic cells show green fluorescence, dead cells show a higher level of green fluorescence and live cells show little or no fluorescence. By using the FL1 channel in FC, other channels are free for multiple labelling experiments.
Product Details
Applications: | Flow Cytometry, Fluorescence microscopy
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Application Notes: | For the detection of apoptotic cells by flow cytometry. |
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Handling: | Protect from light. |
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Shipping: | Blue Ice |
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Long Term Storage: | +4°C |
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Contents: | Annexin V-FITC, SYTOX green dye and binding buffer. An assay protocol is included. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Activators and stimulators of soluble guanylate cyclase counteract myofibroblast differentiation of prostatic and dermal stromal cells: C. Zenzmaier, et al. ; Exp. Cell Res.
338, 162 (2015),
Application(s): Stained human dermal cells to measure apoptosis,
Abstract;