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Cell Counting Kit-F

ALX-850-245-KI01 500 tests 202.00 USD
ALX-850-245-KI02 5x500 tests 738.00 USD
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Fluorometric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages: No radioisotopes, more sensitive than the colorimetric assay, short staining reaction time, no solubilization step, correlates with the [3H]-thymidine incorporation assay.

Product Details

Alternative Name:CCKi-F
Applications:Fluorescence microscopy
Handling:Protect from light.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Technical Info/Product Notes:Principle: The amount of a fluorescent dye, calcein, produced from calcein-AM (3’,6’-Di(O-acetyl)-2’,7’-bis[N,N-bis-(carboxymethyl)aminomethyl]fluorescein . tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Since calcein-AM is highly lipophilic due to the acetoxymethyl groups, it can rapidly permeate into the cytoplasma through the cell membrane. The detection range of the number of viable cells by CCKi-F is from <50 to at least 25,000 cells.
Regulatory Status:RUO - Research Use Only

Product Literature References

Identification of novel pathways and molecules able to down-regulate PHOX2B gene expression by in vitro drug screening approaches in neuroblastoma cells: E. Di Zanni, et al.; Exp. Cell Res. 336, 43 (2015), Application(s): Fluorescence Microscopy , Abstract;

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