The kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-rhodamine 110 (D2R), a reported substrate for members of the caspase family proteases. The caspase substrate D2R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at an excitation of 488nm and an emission of 530nm.
Product Details
| Applications: | Flow Cytometry, Fluorescence microscopy
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| Handling: | Protect from light. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | The kit includes D2R reagent, DTT and the optimal D2R incubation buffer together with an application protocol. |
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| Technical Info/Product Notes: | Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| Regulatory Status: | RUO - Research Use Only |
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