The Quick Apoptotic DNA Ladder Detection Kit offers fast and sensitive detection of DNA fragmentation in apoptotic cells. Unlike other similar kits on the market which require 1-2 days to prepare nucleic acids, the new procedure requires less than 90 minutes to prepare chromosomal DNA in a single tube, with no need for extraction or a column. DNA ladders can easily be visualized by agarose gel electrophoresis. This procedure increases recovery of small fragmented DNA, and therefore improves detection sensitivity.
Figure: Quick detection of DNA ladder in apoptotic cells. Apoptosis was induced in Jurkat cells with camtothecin (2µM) for 0 hours (lane1), 6 hours (lane2) and 12 hours (lane3). Chromosomal DNA was prepared using the Quick Apoptotic DNA Ladder Detection Kit according to the kit instructions. 20µl of each sample was electrophoresed on a 1.2% agarose/EtBr gel.
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Product Details
| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Hepatic CYP3A suppression by high concentrations of proteasomal inhibitors: a consequence of endoplasmic reticulum (ER) stress induction, activation of RNA-dependent protein kinase-like ER-bound eukaryotic initiation factor 2alpha (eIF2alpha)-kinase (PERK) and general control nonderepressible-2 eIF2alpha kinase (GCN2), and global translational shutoff: P. Acharya, et al.; Mol. Pharmacol.
76, 503 (2009),
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