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Caspase-9 fluorometric assay kit

ALX-850-224-KI01 25 tests 314.00 USD
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Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate LEHD-AFC.

Product Details

Alternative Name:ICE-LAP-6, Mch6
Applications:Fluorescent detection
Activity assay
Handling:Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening. Protect from light.
Shipping:Blue Ice
Long Term Storage:-20°C
Contents:25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl LEHD-AFC Substrate (1mM), 100µl DTT (1M).
Technical Info/Product Notes:LEHD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity.

Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
UniProt ID:P55211
Regulatory Status:RUO - Research Use Only

Product Literature References

Stillage Waste from Strawberry Spirit Production as a Source of Bioactive Compounds with Antioxidant and Antiproliferative Potential: C. Spagnuolo, et al.; Antioxidants 12, 421 (2023), Abstract;
Multifunctional thiosemicarbazones targeting sigma receptors: in vitro and in vivo antitumor activities in pancreatic cancer models: M. Niso, et al.; Cell. Oncol. (Dordr.) 44, 1307 (2021), Abstract;
Cadmium nitrate-induced neuronal apoptosis is protected by N-acetyl-l-cysteine via reducing reactive oxygen species generation and mitochondria dysfunction: C.Y. Lee, et al.; Biomed. Pharmacother. 108, 448 (2018), Abstract;
Caspase activation by a zinc-oxide eugenol-based root-canal sealer in cementoblasts: F.M. Huang, et al.; J. Dent. Sci. 10, 338 (2015), Application(s): Fluorometric Assay using murine cementoblasts ,
Synergistic anti-myeloma activity of the proteasome inhibitor marizomib and the IMiD immunomodulatory drug pomalidomide: D.S. Das, et al.; Br. J. Haematol. 171, 798 (2015), Abstract; Full Text

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