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Caspase-8 fluorometric assay kit

ALX-850-222-KI01 25 tests 206.00 USD
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Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate IETD-AFC.

Product Details

Alternative Name:FLICE, Mch5, MACH
Applications:Fluorescent detection
Activity assay
Handling:Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening. Protect from light.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Contents:25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl IETD-AFC Substrate (1mM), 100µl DTT (1M).
Technical Info/Product Notes:IETD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-8 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.

Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
UniProt ID:Q14790
Regulatory Status:RUO - Research Use Only

Product Literature References

Modulation of mutant Huntingtin aggregates and toxicity by human myeloid leukemia factors: M. Banerjee, et al.; Int. J. Biochem. Cell Biol. 10, 82 (2016), Abstract;
Caspase activation by a zinc-oxide eugenol-based root-canal sealer in cementoblasts: F.M. Huang, et al.; J. Dent. Sci. 10, 338 (2015), Application(s): Fluorometric Assay using murine cementoblasts ,
cCMP causes caspase-dependent apoptosis in mouse lymphoma cell lines: S. Wolter, et al.; Biochem. Pharmacol. 98, 119 (2015), Abstract;
Synergistic anti-myeloma activity of the proteasome inhibitor marizomib and the IMiD immunomodulatory drug pomalidomide: D.S. Das, et al.; Br. J. Haematol. 171, 798 (2015), Abstract; Full Text

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