Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate IETD-AFC.
Product Specification
| Alternative Name: | FLICE, Mch5, MACH |
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| Applications: | Fluorescent detection
Activity assay
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| Handling: | Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening. Protect from light. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl IETD-AFC Substrate (1mM), 100µl DTT (1M). |
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| Technical Info/Product Notes: | IETD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-8 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.
Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | Q14790 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Modulation of mutant Huntingtin aggregates and toxicity by human myeloid leukemia factors: M. Banerjee, et al.; Int. J. Biochem. Cell Biol.
10, 82 (2016),
Abstract;
Caspase activation by a zinc-oxide eugenol-based root-canal sealer in cementoblasts: F.M. Huang, et al.; J. Dent. Sci. 10, 338 (2015), Application(s): Fluorometric Assay using murine cementoblasts ,
cCMP causes caspase-dependent apoptosis in mouse lymphoma cell lines: S. Wolter, et al.; Biochem. Pharmacol.
98, 119 (2015),
Abstract;
Synergistic anti-myeloma activity of the proteasome inhibitor marizomib and the IMiD immunomodulatory drug pomalidomide: D.S. Das, et al.; Br. J. Haematol.
171, 798 (2015),
Abstract;
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