Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate IETD-pNA.
Product Details
| Alternative Name: | FLICE, Mch5, MACH |
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| Applications: | Colorimetric detection
Activity assay
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| Handling: | Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl IETD-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution Buffer. |
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| Technical Info/Product Notes: | The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.
Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | Q14790 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Ruxolitinib combined with vorinostat suppresses tumor growth and alters metabolic phenotype in hematological diseases: M. Civallero, et al.; Oncotarget
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Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells: C.C. Chen, et al.; Drug Des. Devel. Ther.
8, 459 (2014),
Abstract;
Full Text
Antidiabetic drug metformin alleviates endotoxin-induced fulminant liver injury in mice: H. Yuan, et al.; Int. Immunopharmacol.
12, 682 (2012),
Abstract;
Therapeutic benefits of the group B3 vitamin nicotinamide in mice with lethal endotoxemia and polymicrobial sepsis: H. Yuan, et al.; Pharmacol. Res.
65, 328 (2012),
Abstract;
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