Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate WEHD-pNA.
Product Specification
| Alternative Name: | ICH-3, Protease TY, ICErel-III |
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| Applications: | Colorimetric detection
Activity assay
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| Handling: | Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light. Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl WEHD-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution Buffer. |
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| Technical Info/Product Notes: | The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | P51878 |
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